不同口器昆虫取食对烟草信号分子及次生代谢物的影响  

Effects of different insect mouthparts feeding on tobacco leaf's signal molecules and secondary metabolite contents

作　　者：张艳敏[1] 白建保[2] 刘剑君[3] 陈翔[1] 周晓峰[1] 程新胜[1] 

机构地区：[1]中国科学技术大学烟草与健康研究中心 [2]中国烟草总公司职工进修学院,郑州市金水区鑫苑路7号450008 [3]河南省烟草公司郑州市公司,郑州市中原区工人路10号450007

出　　处：《烟草科技》2017年第8期16-22,共7页Tobacco Science & Technology

基　　金：河南省烟草公司科技项目"烟粉虱防治的关键技术研究与示范"(20130011)

摘　　要：为揭示不同口器昆虫取食对烟草体内信号分子及次生代谢物的影响,利用烟蚜(Myzus persicae,刺吸式口器)和斜纹夜蛾(Spodoptera litura,咀嚼式口器)取食烟草,测定取食后第3、6、10和15天烟叶中生物碱和蔗糖酯(Sucrose ester,SE)的含量,以及取食后第3、6、9和12小时烟叶与根部一氧化氮(Nitric oxide,NO)和茉莉酸(Jasmine acid,JA)的含量。结果表明:(1)烟蚜和斜纹夜蛾取食烟株后,烟叶中的降烟碱、烟碱和总生物碱含量均呈先上升后下降的趋势,在第6天达到最高。烟蚜取食后,烟碱、降烟碱和总生物碱的含量分别较对照增加89.80%、27.92%和82.20%;斜纹夜蛾取食后,烟叶中的烟碱、降烟碱和总生物碱的含量分别较对照增加84.06%、34.31%和79.15%。(2)烟蚜和斜纹夜蛾取食烟株后,烟叶中的蔗糖酯含量均在第10天达到最高,分别较对照增加60.14%和65.90%。(3)烟蚜和斜纹夜蛾取食烟株后,NO含量在叶部和根部均呈先增加后降低的趋势,9 h后达到最高。处理组叶部与根部的JA含量较对照显著升高,叶部JA含量的变化趋势为先上升后下降,根部则呈持续上升的趋势。To investigate the effects of insects with different mouthparts feeding on tobacco leaves on the signal molecules and secondary metabolites in tobacco, Myzus persicae （with a piercing-sucking mouthpart） and Spodoptera litura （with a chewing mouthpart） were fed on tobacco leaves. The contents of alkaloids and sucrose ester （SE） in tobacco leaves were determined 3, 6, 10, and 15 days after feeding and the contents of nitric oxide （NO） and jasmine acid （JA） in leaves and roots were determined 3, 6, 9, and 12 hours after feeding. The results showed that： After M. persicae and S. litura feeding, 1） the contents of nicotine, nornicotine and total alkaloids increased first, peaked on the 6th day and then decreased. Comparing with the control, the contents of nicotine, nornicotine and total alkaloids in tobacco leaves raised by 89.80%, 27.92% and 82.20% after M. persicae feeding, and by 84.06%, 34.31% and 79.15% after S. litura feeding, respectively; 2） the content of SE in tobacco leaves reached its highest on the 10th day and increased by 60.14% and 65.90% comparing with the control, respectively; 3） the contents of NO in leaves and roots increased first, peaked at the 9th hour and then decreased. The contents of JA in leaves and roots increased significantly. In leaves, JA increased first and then decreased, while JA continued to raise in roots.

关 键 词：烟草 烟蚜 斜纹夜蛾 次生代谢物 信号分子 口器 

分 类 号：S43[农业科学—农业昆虫与害虫防治] TS411[农业科学—植物保护]