一测多评法结合面积归一化法测定西红花中西红花苷类成分  被引量:11

Determination of crocins in Croci Stigma by quantitative analysis of multi-components with single marker and area normalization method

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作  者:周桂芬[1] 姚冲[2] 钱晓东[2] 李丽琴[2] 李晓红[1] 

机构地区:[1]浙江中医药大学,杭州310053 [2]湖州市中心医院,湖州313003

出  处:《药物分析杂志》2017年第8期1524-1529,共6页Chinese Journal of Pharmaceutical Analysis

基  金:国家自然科学基金资助项目(81403032;31600255);浙江省公益技术应用研究项目(2017C32069;2015C33272);浙江省自然科学基金青年基金项目(LQ15H280001);浙江省中医药科技计划科学研究基金项目(2015ZB112)

摘  要:目的:采用一测多评法结合面积归一化法,对西红花药材中西红花总苷进行质量控制,解决HPLC法测定含量时对照品不易获得的问题。方法:以西红花苷-Ⅰ为内参物,建立西红花苷-Ⅰ对西红花苷-Ⅱ的相对校正因子,利用相对校正因子计算西红花苷-Ⅱ的含量,实现一测多评;并与中国药典采用的外标法测定的32批西红花中西红花苷-Ⅰ和苷-Ⅱ含量进行比较,以验证一测多评法的可行性。同时以西红花苷-Ⅰ为对照,面积归一化法检测西红花中总西红花苷的含量。结果:西红花苷-Ⅰ相对西红花苷-Ⅱ的相对校正因子重复性好,一测多评法测定的32批西红花中西红花苷-Ⅱ的含量范围为1.2%~8.2%,与外标法测定的结果基本一致。面积归一化法测定的总西红花苷含量范围为7.1%~28.7%,与UV法测定的总西红花苷含量无显著性差异。结论:一测多评法结合面积归一化法测定西红花苷含量,该方法准确可靠,简便可行,能更合理地反映西红花的内在质量,可用于西红花中西红花苷类成分的质量控制。Objective: To establish a method for determination of total crocins in Croci Stigma so as to overcome difficulties in the obtainment of reference compound. Methods: Crocin- I was used as reference to establish its relative correction factor ( RCF ) to crocin- II in order to perform quantitative analysis of multi-components with single marker( QAMS ). The contents of crocin- II in 32 batches of Croci Stigma were determined with RCF and external standard method ( ESM ) , respectively. The accuracy and feasibility of quantitative analysis of QAMS were evaluated by comparison of the data based on ESM and QAMS. Meanwhile, crocin- I was also used as the reference for determining the contents of total crocins by using area normalization method ( ANM ). Results: RCF had a good repeatability. The contents of crocin- 11 in 32 batches of Croci Stigma, determined by QAMS, were 1.2%-8.2%. and they were of no significant differences compared with determined by ESM. The contents of total crocins determined by ANM were 7.1%-28.7%, and they were consistent with those determined by UV. Conclusion: The presented method, QAMS combined with ANM, is accurate, simple and feasible, which can be used as a quality control method for Croci Stigma as crocins.

关 键 词:西红花 藏红花 西红花苷 胡萝卜素类化合物 质量控制 外标法(ESM) 一测多评(QAMS) 面积归一化法(ANM) 

分 类 号:R917[医药卫生—药物分析学]

 

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