反相高效液相色谱法与荧光偏振免疫法测定血浆卡马西平浓度比较  

Comparison of Reversed Phase High Performance Liquid Chromatography and Fluorescence Polarization Immunoassay for the Determination of Plasma Concentration of Carbamazepine

作　　者：白万军[1] 孙晓利[2] 安静[1] 宋浩静[1] 董占军[1] 

机构地区：[1]河北省人民医院药学部,石家庄050051 [2]河北医科大学第四医院药学部,石家庄050017

出　　处：《医药导报》2017年第9期1043-1046,共4页Herald of Medicine

基　　金：河北省2013年医学科学研究重点课题计划项目(20130409);河北省2015年医学科学研究重点课题计划项目(20150133)

摘　　要：目的比较反相高效液相色谱(RP-HPLC)法与荧光偏振免疫(FPIA)法测定血浆卡马西平浓度的差异性和相关性。方法收集卡马西平血浆样本50例,分别采用RP-HPLC和FPIA进行测定,采用配对t检验比较两者均数的差异;Bland-Altman进行一致性分析;Deming回归法进行相关性分析。结果 FPIA法的测定结果高于RP-HPLC法,两种测定方法均数之间差异有统计学意义(P<0.05),一致性较差;Deming回归分析显示两种方法之间存在良好的相关性,回归方程为:CFPIA=1.195 3CRP-HPLC-0.144 0,Pearson相关系数为0.968 5。结论在制定卡马西平个体化给药方案时应充分考虑不同检测方法的影响,正确解读检测结果,提高个体化用药水平。Objective To evaluate the correlation and difference of reversed phase high performance liquid chromatography （ RP-HPLC ） and fluorescence polarization immunoassay （ FPIA） on determining serum concentration of carbamazepine. Methods Fifty serum samples were collected, both RP-HPLC and FPIA methods were employed to determine the concentration of carbamazepine.The results were analyzed by paired t test, Bland-Altman and Deming regression methods, respectively. Results The results of measuring 50 samples by the two methods showed that FPIA datas were significantly higher than RP-HPLC datas,and there was statistically significant difference（ P 〈0. 05 ） and poorer consistency between two methods; There was good correlation between carbamazepine concentrations determined by the two methods. Deming regression equation was CmA = 1.195 3 CRP-HPLC-0.144 0, and Pearson correlation coefficient was 0.968 5. Conclusion Clinicians should pay more attention to the difference of carbamazepine concentration determination by different methods when carbamazepine individualized dosage regimen was adjusted according to therapeutic drug monitoring.

关 键 词：卡马西平 色谱法 高效液相 反相 荧光偏振免疫法 血药浓度 

分 类 号：R971.6[医药卫生—药品] R917[医药卫生—药学]