番茄灰霉病病原菌分离鉴定及拮抗菌筛选  

Isolation and Identification of Pathogenic Fungus of Botrytis cinerea and Screening of Antagonistic Bacteria Against Tomato Gray Mold

作　　者：陈哲[1] 黄静[1] 赵佳[1] 梁宏[1] 

机构地区：[1]山西省农业科学院生物技术研究中心,太原030031

出　　处：《生物技术通报》2017年第8期81-87,共7页Biotechnology Bulletin

基　　金：山西省科技自主创新能力提升项目(2015zzcx-22);山西省应用基础研究项目(201601D202062)

摘　　要：灰葡萄孢菌(Botrytis cinerea)引起的番茄灰霉病是番茄生产上的重要病害。从番茄果实表面成功分离了1株真菌BC2016-2,经过鉴定确定其为灰霉病的病原菌——灰葡萄孢菌;以灰葡萄孢菌BC2016-2为指示菌,筛选到了3株具有明显抑制作用的菌株,分别为解淀粉芽孢杆菌(Bacillus amyloliquefacien)CM3、巨大芽孢杆菌(Bacillus megaterium)Y-30和解淀粉芽孢杆菌Y-48。盆栽实验结果显示,菌株CM3、Y-30和Y-48对灰葡萄孢菌BC2016-2引起的番茄灰霉病的防治效果分别为65.58%、54.1%和72.13%。It is well known that Botrytis cinerea is one of the most geographically widespread plant pathogen on tomato. A fungusBC2016-2 was successfully isolated from the tomato surface and identified as B. cinerea,a pathogen of tomato gray mold. Then,three strainswith obvious inhibitory effects were screened using B. cinerea BC2016-2 as indicator fungus,and they were Bacillus amyloliquefacien CM3,Bacillus megaterium Y-30 and Bacillus amyloliquefaciens Y-48. The greenhouse pot experiment showed that the control effects of CM3,Y-30 and Y-48 on the tomato gray mold caused by B. cinerea BC2016-2 were 65.58%,54.1% and 72.13%,respectively.

关 键 词：灰葡萄孢菌 解淀粉芽孢杆菌 巨大芽孢杆菌 生物防治 

分 类 号：S436.412.13[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]