盐胁迫下胡杨和合作杨悬浮细胞端粒酶与抵御氧化损伤的关系  

Telomerase Activity in Relation to Oxidative Damage Resistance in Cells of Populus euphratica and ×P. simonii P. pyramibalis cv Under Salt Stress

作　　者：吴晓飞[1] 王瑾瑜[2] 张徐俞[1] 孙玉萍[1] 陈玉珍[1] 卢存福[1] 

机构地区：[1]北京林业大学生物科学与技术学院林木育种国家工程实验室教育部林木花卉遗传育种重点实验室,北京100083 [2]清华大学分析测试中心,北京100084

出　　处：《生物技术通报》2017年第8期111-119,共9页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31270737);高等学校学科创新引智计划(B13007);长江学者和创新团队发展计划(IRT13047);北京市自然科学基金项目(6112016)

摘　　要：胡杨是我国西北干旱盐碱荒漠和戈壁地带唯一能形成森林的高大乔木树种,具有较高抗盐性。旨在探究盐胁迫下细胞端粒酶活性变化与氧化损伤的关系,以抗盐的胡杨和盐敏感的合作杨悬浮细胞为材料,检测了Na Cl盐胁迫条件下胡杨和合作杨细胞生长、超氧阴离子自由基与丙二醛含量以及端粒酶活性的变化。结果表明,在正常生长条件下,胡杨和合作杨细胞符合S型生长曲线,且合作杨细胞的生长量高于胡杨细胞;在盐胁迫<100 mmol/L时胡杨在培养7 d内细胞活力高于对照(0 mmol/L),即使盐浓度达到300 mmol/L时15 d胡杨细胞依然保持一定的细胞活力,而较低盐处理对合作杨细胞活力影响即较大,当盐浓度达到300 mmol/L时合作杨在第5 d细胞活力已接近零;与合作杨相比,低浓度盐胁迫(100 mmol/L)使胡杨细胞内超氧阴离子自由基含量增加,端粒酶活性较高,而合作杨丙二醛含量显著增加;低浓度(<20 mmol/L)H2O2诱导了胡杨细胞端粒酶活性,合作杨的变化则不显著,但高浓度(>200 mmol/L)Na Cl和高浓度(50 mmol/L)H_2O_2可能造成了胡杨和合作杨氧化损伤,端粒酶活性降低。抗盐性强的胡杨细胞端粒酶对抵御细胞内氧化损伤具有一定作用。Populus euphratica is an arbor that grows in drought and saline area in Northwest China. This work aims to explore the relationof telomerase activity and oxidative damage resistance. Using Populus euphratica and P. simonii×P. pyramibalis cv callus cells as materials,superoxide anion free radical levels,malondialdehyde contents and telomerase activity under salt stress were investigated. Results indicatedthat the fresh growth were consistent with sigmoid growth curve in both P. euphratica and P. simonii × P. pyramibalis cv cells,and the growthof P. simonii × P. pyramibalis cv was higher than that of P. euphratica. Compared to the control（0 mmol/L）,the cell activity of P. euphraticaincreased when cultured for 7 days under the treatment of 100 mmol/L Na Cl,and maintained at a certain level under the treatment of 300 mmol/L Na Cl for 15 days. However,the cell activity of P. simonii × P. pyramibalis cv was affected under low Na Cl concentration,and approximatedto zero when cultured for 5 days under 300 mmol/L Na Cl. Under the treatment of 100 mmol/L Na Cl,the content of superoxide anion free radicaland telomerase activity in P. euphratica cells increased compared to P. simonii × P. pyramibalis cv cells,while the malondialdehyde content in P. simonii × P. pyramibalis cv cells rose significantly. The telomerase activity in P. euphratica cells was much higher than that in P. simonii×P. pyramibalis cv cells when cultured in 300 mmol/L Na Cl. The telomerase activity in P. euphratica cells increased but no significant change in P. simonii×P. pyramibalis cv cells under the low concentration of H2O2;high concentration of Na Cl or H2O2 caused the oxidative damages to bothP. euphratica and P. simonii × P. pyramibalis cv cells,resulting in the decrease of telomerase activity. Experimental results suggest that thetelomerase can play certain role in defensing oxidative damage.

关 键 词：胡杨 合作杨 盐胁迫 端粒酶 氧化损伤 

分 类 号：S792.11[农业科学—林木遗传育种]