芍药内酯苷对大鼠坐骨神经雪旺细胞的保护作用研究  

Study on Albiflorin Activity in Protection of Schwann Cells in Rats' Sciatic Nerve

作　　者：王东超[1,2,3] 魏颖[1,2,3] 高佳琪[1,2,3] 刘一冰[1] 屈玲霞 刘永巧[1,2,3] 徐暾海[1,2,3] 刘铜华[2,3] 

机构地区：[1]北京中医药大学中药学院,北京100029 [2]北京中医药大学中医养生学教育部重点实验室,北京100029 [3]北京中医药大学中医养生学北京市重点实验室,北京100029

出　　处：《世界科学技术-中医药现代化》2017年第5期780-785,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家重大新药创制科技重大专项(2012ZX09102201-001):治疗糖尿病周围神经病变药物糖痹康临床前研究;负责人:刘铜华;北京中医药大学创新团队-中医药干预糖尿病及其并发症研究团队(2011-CXTD-19);负责人:刘铜华

摘　　要：目的:探讨糖痹康颗粒中主要成分芍药内酯苷对大鼠坐骨神经雪旺细胞(Schwann Cells,SCs)的影响作用。方法:本实验通过不同浓度的葡萄糖对大鼠坐骨神经雪旺细胞的影响的研究,建立了以150 mmol·L^(-1)D-葡萄糖,培养时间为48 h的细胞氧化应激模型,通过设立芍药内酯苷高中低组(100μM、20μM、4μM),模型组,维生素C组(100μM),正常组,利用流式细胞仪定量检测细胞内ROS含量和荧光显微镜定性观察细胞内ROS状态以及CCK-8检测细胞活性。结果:采用CCK-8法检测细胞增殖情况,48 h后,模型组与正常组有明显的差异P<0.01;芍药内酯苷给药组与模型组相比,P<0.01;芍药内酯苷可以促进雪旺细胞增殖。通过检测ROS荧光含量,发现芍药内酯苷100、20、4μM与模型组(Glu 150 mM)相比,各组P<0.01,表明芍药内酯苷可以减轻雪旺细胞内ROS,缓解氧化应激状态。结论:芍药内酯苷可以通过增加雪旺细胞增殖,改善氧化应激状态对雪旺细胞产生保护作用。This paper was aimed to study the albiflorin activity of Tang-Bi-Kang（TBK） granules in protecting Schwanncells（SCs） of rat＇s sciatic nerve. The establishment of SCs oxidative stress model was the condition of 150 mmol×L^-1 D-glucose with different concentrations. And the incubation time was 48 h. The experiment groups were the high-dose,middle-dose and low-dose（100 μM, 20 μM, 4 μM） albiflorin group, the model group, the vitamin C（100 μM） group,and the normal group. Flow cytometry was used to detect the content of ROS in SCs. Fluorescence microscope was usedto observe the condition of ROS fluorescence in SCs. And CCK-8 was used to detect the cell activity. The results showedthat by using CCK-8 to detect cell proliferation, after 48 h, there was a significant difference between the model groupand the normal group（P〈0.01）; the albiflorin group compared with the model group（P〈0.01）. It indicated that albiflorincan promote the proliferation of SCs. Detecting the ROS fluorescence content, it showed that compared with the modelgroup（Glu 150 m M）, the 100 μM, 20 μM, and 4 μM albiflorin group, it was P〈0.01 for each group. It showed thatalbiflorin could relieve the ROS in SCs and alleviate oxidative stress. It was concluded that albiflorin can increase theproliferation of SCs and improve the state of oxidative stress with the protection of SCs.

关 键 词：糖痹康颗粒 芍药内酯苷 雪旺细胞 神经保护 

分 类 号：R285.5[医药卫生—中药学]