生脉注射液对实验性心肌缺血Beagle犬生理指标及血清C-反应蛋白、NO水平的影响  

Effects of Shengmai Injection on Physiological Indices, C-reactive Protein and NO Levels in Experimental Beagle Dogs with Myocardial Ischemia

作　　者：甘雨[1] 包玉龙[1] 张宏[1] 王菲[1] 范英兰[1] 乔敏[1] 刘小虎[1] 夏素霞[1] 李国信[1] 

机构地区：[1]辽宁省中医药研究院,沈阳110034

出　　处：《世界科学技术-中医药现代化》2017年第5期831-836,共6页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家"十二五"中药新药临床评价研究技术平台建设(2012ZX09303-017-001):中药新药Ⅰ期临床关键技术和方法的研究;负责人:李国信

摘　　要：目的:探讨生脉注射液对心肌缺血Beagle犬血压、心率、心电图及血清C-反应蛋白、NO水平的影响。方法:30只Beagle犬随机分为5组,即空白对照组、模型对照组、生脉注射液小剂量组(0.80 m L·kg^(-1))、生脉注射液大剂量组(3.20 m L·kg^(-1))和单硝酸异山梨酯组(0.07 m L·kg^(-1))。采用皮下注射盐酸异丙肾上腺素制备心肌缺血模型,同时给予药物治疗。记录给药后1、6、12、24 h,犬血压、心率和心电图变化,并检测血清C-反应蛋白和NO含量。结果:与空白对照组比较,模型组Beagle犬平均动脉压(MBP)明显降低(P<0.01),心率(HR)明显加快(P<0.01或P<0.05),心电图ΔST值明显增大(P<0.01),生脉注射液能改善上述症状,但作用趋缓。生脉注射液对血清C-反应蛋白含量降低和NO含量升高有明显的对抗作用,与模型组比较,差异有统计学意义(P<0.01或P<0.05)。结论:生脉注射液能改善心肌缺血Beagle犬生理指标,其机制可能与调节C-反应蛋白代谢,抑制NO分泌有关。This study was aimed to explore the effects of Shengmai（SM） injection on blood pressure（BP）, heart rate（HR）,electrocardiogram（ECG）, and serum C-reactive protein（CRP） and NO levelin Beagle dogs with myocardial ischemia（MI）. A total of 30 Beagle dogs were divided into 5 groups, which were the blank control group, model control group, SM injection low-dose group（0.80 m L·kg^-1）, SM injection high-dose group（3.20 m L·kg^-1） and isosorbidemononitrate group（0.07 m L·kg^-1）. The MI model was established by the subcutaneous injection of isoprenaline hydrochloride, and the drug treatments were given at the same time. At 1 h, 6 h, 12 h, 24 h after administration, changes of BP, HR and ECG were recorded; serum CRP and NO content were detected. The results showed that compared with the blank control group, the mean arterial pressure（MBP） was significantly decreased（P〈0.01）, HR was significantly increased（P〈0.01, or P〈0.05）,the ECGΔST value was significantly increased（P〈0.01） in the model group. SM injection could improve symptoms mentioned above, but the effectswereslow. SM injection had a significant antagonistic effect on the decreasing of CRP content and the increasing of NO content, compared with the model group. The difference was statistically significant（P〈0.01, or P〈0.05）. It was concluded that SM injection could improve the physiological indices of Beagle dogs with MI,which might be related to the regulation of CRP and the inhibition of NO secretion.

关 键 词：生脉注射液 心肌缺血 生理指标 C-反应蛋白 一氧化氮 

分 类 号：R965.1[医药卫生—药理学]