四川不同产地厚朴中挥发油及多糖含量分析  

Analysis of volatile oil and polysaccharide in Houpo from different habitats

作　　者：董佳悦 刘美琳[1] 任波[1] 张梅[1] 

机构地区：[1]成都中医药大学药学院,中药材标准化重点实验室,中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中药与临床》2017年第2期22-26,52,共6页Pharmacy and Clinics of Chinese Materia Medica

基　　金：四川省科技计划项目“中药种质资源库建设关键技术研究及示范”(项目编号:2014SZ0156)

摘　　要：目的:分析比较四川不同产地厚朴中挥发油和多糖的含量,鉴别挥发油组成,为川厚朴产区优质种质资源筛选及质量标准研究提供参考。方法:采用《中国药典》(2015年版)挥发油测定法甲法测定厚朴中挥发油含量,并运用GC-MS法鉴别挥发油组成;采用可见分光光度法测定厚朴中多糖的含量。结果:不同产地厚朴挥发油、多糖含量分别为0.18%~0.40%及5.47%~21.51%;从10个产地厚朴挥发油中共鉴定出93个化学成分,其中10种为共有成分。结论:四川境内不同产地厚朴中挥发油在成分及含量上存在一定的差异,多糖含量差异较大。Objective： The contents of volatile oil and polysaccharide in Houpo from different habitats in Sichuan Province were analyzed. The chemical components of the volatile oil were identified to provide reference for the selection of high quality germplasm resources and quality standards of Houpo in Sichuan province. Method： The volatile oil content of Houpo was detected by the method recorded in ＂Chinese Pharmacopoeia＂ （2015 edition） and chemical components were identified by GC- MS. Polysaccharide contents were detected by visible spectrophotometry. Result： The content of volatile oil of Houpo was 0.18% 0.40% and the polysaccharide content was 5.11%-21.51%. Ninety-three compounds were identified from volatile oil of Houpo from ten producing areas in Sichuan province. And ten compounds were found in all the samples. Conclusion： The content and composition of volatile oil in Houpo from different area of Sichuan province are different, and polysaccharide content is varied in large range.

关 键 词：川厚朴 挥发油 多糖 GC-MS分析 含量测定 

分 类 号：R282[医药卫生—中药学]