促兔眼蓝莓生长菌根真菌的分离与鉴定  

Isolation and identification of mycorrhizal fungi for the promoting growth of Vaccinium Ashei

作　　者：赵磊[1] 刘伟[1] 傅翔[1] 单晓笛 张书祥[1] 蒋光月[2] 黄蓓[1] ZHAO Lei LIU Wei FU Xiang SHAN Xiao-di ZHANG Shu-xiang JIANG Guang-yue HUANG Bei(School of Life Science, Anhui University, Hefei 230601 Anhui Academy of Agricultural Sciences, Hefei 230031, China)

机构地区：[1]安徽大学生命科学学院,合肥230601 [2]安徽省农业科学院,合肥230031

出　　处：《生物学杂志》2017年第4期116-120,共5页Journal of Biology

基　　金：安徽省科技厅科技攻关项目(No.1301032140)

摘　　要：利用优势菌根真菌提高弱根系兔眼蓝莓对营养元素的吸收,促进兔眼蓝莓植株的生长。从皖南地区兔眼蓝莓菌根分离得到的9种真菌进行了功能试验:通过兔眼蓝莓幼苗接种试验,观察菌根真菌对兔眼蓝莓幼苗存活率及株高的影响;盆栽兔眼蓝莓植株接种试验观察菌根真菌侵染情况,检测其对兔眼蓝莓植株叶片N、P、K及叶绿素含量变化的影响。筛选得到4种对兔眼蓝莓生长有显著促进作用的菌株。利用形态学观察、DNA测序及构建系统发育树,分析发现其中3种属于毛霉属卷枝毛霉种(Mucor circinelloides),其亚种水平有差异,另外1种属于毛霉属总状枝毛霉(Mucor racemosus),其相似率均大于99%。毛霉属卷枝毛霉种及总状枝毛霉种对兔眼蓝莓营养元素的吸收和植株的生长具有明显的促进作用。Nine mycorrhizal fungi were isolated from the roots of Vaccinium Ashei in southern Anhui Province.The inoculation experiments for the mycorrhizal fungi were performed to detect the survival rate and the height in the shoots and to test the nutrient elements and chlorophyll content in plants of blueberry.four Fungi strains were found as dominant strains in promoting growth of Vaccinium Ashei.Through morphological observation and molecular identification method, we found that three strains belong to families Mucor species Mucor circinelloides, and the different among them may be in subspecies level.Another species belongs to families Mucor species Mucor racemosus, and the similarity between them was greater than 99%.The mycorrhizal fungi from families Mucor species Mucor circinelloides and Mucor racemosus can significant promote the nutrient absorption and growth of Vaccinium Ashei.

关 键 词：兔眼蓝莓 菌根真菌 接种试验 ITS序列 分子鉴定 

分 类 号：Q93-331[生物学—微生物学]