microRNA-145调控大鼠骨髓间充质干细胞成骨分化的实验研究  

The study of microRNA-145(miR-145) modulating osteogenesis differentiation of rat bone marrow mesenchymal stem cells(rBMMSC)

作　　者：沈树平 陆亚倩 郑旸[2] 张玮[1] 朱庆萍[1] SHEN Shuping LU Yaqian ZHENG Yang ZHANG Wei ZHU Qingping.(Jiangsu Key Laboratory of Oral Diseases, Nanfing Medical Universit Special Clinical Department, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, China)

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院特诊科,江苏南京210029 [2]南京医科大学口腔疾病研究江苏省重点实验室,江苏南京210029

出　　处：《口腔医学》2017年第8期693-697,共5页Stomatology

基　　金：国家自然科学基金青年项目(81500823)

摘　　要：目的探讨microRNA-145在大鼠骨髓间充质干细胞成骨分化过程中的作用。方法取3周龄雄性大鼠胫骨骨髓间充质干细胞,分为阴性对照组与microRNA-145下调组,分别感染慢病毒,观察microRNA-145对大鼠骨髓间充质干细胞在细胞增殖、细胞周期、凋亡和成骨分化方面的影响。结果 microRNA-145下调表达组(p Lv3-(anti)miR-145)与阴性对照组(p Lv3-Negative control)相比,大鼠骨髓间充质干细胞的增殖、细胞周期和凋亡无统计学差异(P>0.05);microRNA-145下调表达组成骨相关基因Sema3A、OPG、Runx2及Osterix/SP7表达均明显高于阴性对照组,且microRNA-145下调组茜素红染色范围也大于阴性对照组。结论 microRNA-145下调表达可以促进大鼠骨髓间充质干细胞的成骨分化,但对其细胞增殖、细胞周期和凋亡无明显影响。Objective To investigate the effect of miR-145 on the osteogenesis differentiation of rBMMSC. Methods Rat shin bone marrow mesenchymal stem cells collected from 3-week male rats were divided into two groups, pLv3-Negative control group and pLv3- （anti） miR-145 group, transfected with lentivirus. The effects of miR-145 on cell proliferation, apoptosis, growth cycle and osteogenesis differentiation of rBMMSC were observed. Results There was no difference in cell proliferation, growth cycle and apopto- sis between pLv3-Negative control group and pLy3-（anti） miR-145 group. The expressions of Sema3A, OPG, Runx2 and Osterix/SP7 in pLv3-（anti）miR-145 group were significantly higher than pLv3-Negative control group. The area of Alizarin red staining in pLv3- （anti） miR-145 group was also bigger than pLv3-Negative control group. Conclusion Inhibition of miR-145 expression can enhance osteogenesis differentiation of rBMMSC, but it has no obvious effect on cell proliferation, growth cycle and apoptosis of rBMMSC.

关 键 词：microRNA-145 大鼠骨髓间充质干细胞 成骨分化 

分 类 号：R349.69[医药卫生—基础医学]