盐生植物内生解烃细菌的分离及其特性  

Isolation and Characteristics of Hydrocarbon-degrading Endophytic Bacteria from Halophytic Vegetation

作　　者：鲁红[1,2] 吴涛[1,2] 姚志刚[1] 谢文军[1,2] 刘俊华[2] 段代祥[1] 张孝霖[1] 

机构地区：[1]滨州学院生物工程学院,山东省黄河三角洲野生植物资源开发利用工程技术研究中心,山东滨州256600 [2]滨州学院,山东省黄河三角洲生态环境重点实验室,山东滨州256600

出　　处：《安徽农学通报》2017年第16期18-20,27,共4页Anhui Agricultural Science Bulletin

基　　金：山东省高等学校科技计划项目,J14LE05;国家级大学生创新训练项目,201410449021;山东省自然科学基金项目,ZR2013DM001

摘　　要：为获得盐生植物内生解烃细菌,该研究以黄河三角洲石油污染地区健康生长的盐生植物白茅、芦苇、蒙古鸦葱为材料,通过柴油平板培养实验分离得到22株内生解烃细菌。经16S rRNA同源序列分析,对菌株进行了鉴定。通过液体培养实验,研究了菌株对不同烃类物质利用能力。结果表明,22株内生细菌主要为原小单胞菌属、短小杆菌属、微杆菌属、假单胞菌属、芽孢杆菌属、沙雷氏菌属、考克氏菌属、不动杆菌属、红小梨形菌属和节细菌属,其中假单胞菌属和芽孢杆菌属菌株最多。分离出22株内生解烃细菌能降解直链烷烃的最多,能降解多环芳烃种类最少,其中部分内生解烃细菌既可以利用烷烃又可以利用芳香烃。To obtain efficient hydrocarbon-degrading endophytic bacteria from halophytic vegetation, 22 endophytic bacteria strains which can grow in mineral liquid media amended with diesel oil as the sole carbon sources were iso- lated from lmperata cylindrica （L.） Beauv., Phragmites australias Trin., and Scorzonera mongolica Maxim.growing in a heavily saline and alkali, crude oil-contaminated environment in the Yellow River Delta, an important base of pe- troleum production in China.According to its 16S rRNA sequence analysis, these strains were identified.Moreover, liquid incubation experiment was conducted to clarify its characteristics of degrading efficiency for various hydrocar- bons.Sequence analysis revealed that 22 strains were members of 10 genera （Promicromonospora, Curtobacterium, M icrobacterittm , Pseudomonas , Bacillus, Serratia , Kocuria , Acinetobacter , Rhodopirellula , Arthrobacter ） , and the dom- inant genera were Pseudomonas and Bacillus.Most of endophytic bacteria strains can degrade straight-chain alkanes, polycyclic aromatic hydrocarbons degradation species were the least.Some endophytic bacteria strains can utilize both alkanes and aromatic hydrocarbons.

关 键 词：石油污染 盐渍化土壤 盐生植物 内生细菌 解烃 

分 类 号：Q936[生物学—微生物学]