骨搬移技术-压应力对成骨细胞OPG/RANKL表达的影响  

Molecular mechanism of removal technology with constant compressive stress on the expression of osteoclast

作　　者：廉永云[1] 逯代锋[1] 周勇[1] 姜明久[1] 孙闯[1] 

机构地区：[1]哈尔滨医科大学附属第四医院骨科,黑龙江哈尔滨150001

出　　处：《哈尔滨医科大学学报》2017年第3期211-213,共3页Journal of Harbin Medical University

基　　金：黑龙江省教育厅科学技术研究项目(12521182)

摘　　要：目的评估恒定压应力对雄性大鼠成骨细胞OPG/RANKL表达的影响。方法恒定压应力下对雄性大鼠成骨细胞加载相同应变频率、不同加载时间以及不同应变频率、不同的加载时间后,经PCR检测OPG/RANKL表达差异。结果加载应力相同,加载时间和频率不同时,A组RANKL具有统计学意义(P<0.05);而OPG较其它各组差别不大,无统计学意义(P>0.05)。加载时间相同,加载频率与加载压应力不同时,A、B、C三组比值比较对照组OPG、RANKL皆差别很大,具有统计学意义(P<0.05)。结论在加载时间相同的情况下(恒定为4 h)成骨细胞OPG与RANKL的比值随着压应变频率和预压应力的增加而升高。Objective To evaluate the effects of constant compressive stress on osteoblast OPG/RANKL expression in strain frequency at different 1 male rats. Methods The osteoclasts were loaded with the sane oading time and different strain frequency at different loading time of the constant compressive stress. And the expression of OPG/RANKL was detected by PCR. Results The RANKL result of group A with the same constant compressive stress, different loading time and different strain frequency was statistically significant （ P 〈 0. 05 ）. But there was no statistical significance of OPG among the others （ P 〉 0. 05 ）. Both the RANKL and OPG results of control group with the different constant compressive stress, the same loading time and the same strain frequency were statistically significant （P 〈 0. 05 ）. Conclusion The new mechanical loading bioreactor pressure oscillation frequency at time of 4 hours after OPG/ RANKL positive adjustment.

关 键 词：恒定压应力 成骨细胞 OPG/RANKL 

分 类 号：R683[医药卫生—骨科学]