浙贝母花脂肪油成分和生物活性研究  

Chemical constituents and bioactivities of the fatty acids of fl owers of Fritillaria thunbergii Miq

作　　者：彭伟[1] 黎江华[1] 刘玉杰[1] 吴纯洁[1] 蒋淼[1] 廖博[1] 苏轩艺 

机构地区：[1]成都中医药大学药学院,中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验室省部共建国家重点实验室培育基地,四川成都611137

出　　处：《中药与临床》2017年第3期19-21,68,共4页Pharmacy and Clinics of Chinese Materia Medica

摘　　要：目的:研究浙贝母花脂肪油(CFD)的化学成分和生物活性。方法:采用气质联用(GC-MS)对CFD化学成分进行分析。采用DPPH自由基清除实验评价CFD的抗氧化活性;采用小鼠醋酸扭体实验对CFD镇痛活性进行研究。结果:实验结果表明CFD中主要为不饱和脂肪酸(46.96%)、饱和脂肪酸(16.83%):(9Z,12Z,15Z)-octadeca-9,12,15-trienoic acid(26.55%)、Linoleic acid(20.41%)、Palmitic acid(16.43%)。抗氧化实验结果表明CFD有较强的DPPH自由基清除能力,其半数清除率(EC50)为4.57 mg/m L;醋酸扭体实验结果表明,和对照组小鼠相比,CFD灌胃(100 mg/kg和200mg.kg^(-1))不仅能显著降低小鼠扭体次数(p<0.05,p<0.01),还能延长小鼠扭体潜伏时间(p<0.05,p<0.01)。结论:浙贝母花脂肪油含有丰富的不饱和脂肪酸,并且具有潜在的抗氧化和镇痛活性。Objective： To investigate chemical constituents and bioactivities of fatty acids from the flowers of Fritillaria thunbergii Miq. （CFD）. Method： GC-MS was used to analyze compositions of CFD. DPPH radical scavenging assay was used to evaluate its antioxidant properties, and the acetic acid-induced abdominal writhing test in mice was performed to investigate its antinociceptive effects. Result： The results revealed that main components of CFD were （9Z, 12Z, 15Z）-octadeca-9,12,15-trienoic acid （26.55 %）, linoleic acid （20.41%）, Palmitic acid （16.43 %）, indicating that CFD contained plenty of unsaturated fatty acid （46.96%） and saturated fatty acids （16.83 %）. In addition, results also demonstrated that CFD possessed significant DPPH radical scavenging activity （ECs0 = 4.57 mg/mL）. Furthermore, CFD （100 and 200 mg. kg4） can not only significantly decreased the number of writhing episodes induced by acetic acid （9 〈 0.05, p 〈 0.01）, but also increased the latencies of the writhing （9 〈 0.05, p 〈 0.01）. Conclusion： CFD possess significant anti-oxidant and antinociceptive effects, and unsaturated fatty acid is the main constituent of CFD.

关 键 词：FRITILLARIA thunbergii Miq 贝母花 脂肪油 不饱和脂肪酸 抗氧化 镇痛 

分 类 号：R282[医药卫生—中药学]