高效降解复合菌系的廉价降解培养基筛选  

Screening of the cheap degradation media for an effective azo dye degradation microbial consortium

作　　者：陈燕[1] 肖婷[1] 王园秀[1] 李汉广[1] 沈飞[1] 张庆华[1] 

机构地区：[1]江西农业大学生物科学与工程学院,江西省农业微生物资源开发与利用工程实验室,江西南昌330045

出　　处：《工业水处理》2017年第8期45-49,共5页Industrial Water Treatment

基　　金：国家自然科学基金(31660024);江西省自然科学基金(20151BAB204001);江西省教育厅科技项目(GJJ14297,GJJ150436);江西省杰出青年人才资助计划(20171BCB23044);江西农业大学大学生创新训练计划项目(201610410105)

摘　　要：考察了麸皮、鱼粉、玉米粉、棉籽粉、花生粉、豆粕及尿素对偶氮染料降解复合菌系的生长、产酶及底物降解等方面的影响,从而筛选出最适宜的廉价氮源。研究表明,采用麸皮和鱼粉作为廉价氮源并控制其含氮量的质量比为4∶2时,复合菌系对偶氮染料的降解效果最佳。同时还发现,最佳的廉价培养基(BFS)组合为:麸皮18.76g/L、葡萄糖5.00 g/L、鱼粉3.08 g/L、KH_2PO_4 1.80 g/L、NaH_2PO_4 3.50 g/L、FeCl_3 0.01 g/L、MnSO_4 0.02 g/L、MgSO_4 0.20 g/L、pH 7.2。筛选出的廉价培养基(BFS)成本仅为原蛋白胨染料降解培养基(PDS)成本的8.77%。The on the growth influences of bran, fish meal, corn flour, cotton-seed meal, peanut flour, soybean meal and urea, , enzyme secretion, and substrate degradation of azo dye degradati have been investigated, so as to screen out the most suitable cheap nitrogen source. bran and fish meal are used as cheap nitrogen sources, on microbial consortium, etc. The research shows that when and their nitrogen mass proportion is controlled at 4 ： 2, the degradation effect of the composite microbial consortium on azo dye is the best. It is found that the optimum consti- tution of cheap medium （BFS） is as follows： bran is 18.76 g/L, glucose 5.00 g/L, fish meal 3.08 g/L, KH2PO4 1.80 g/L, NaH2PO4 3.50 g/L, FeCl3 0.01 g/L, MnSO4 0.02 g/L, MgSO4 0.20 g/L, and pH 7.2. Furthermore, it is found that the cost of the screened out cheap medium （BFS） is only 8.77% of the cost of traditional peptone-dye degradation media.

关 键 词：偶氮染料 复合菌系 脱色 偶氮还原酶 廉价培养基 

分 类 号：X703[环境科学与工程—环境工程]