Ultrasound extraction optimization, structural features, andantioxidant activity of polysaccharides from Tricholoma matsutake  

松茸多糖的超声波提取优化、结构特性和抗氧化活性研究（英文）

作　　者：Yun CHEN Xiu-ju DU Yang ZHANG Xin-hua LIU Xuan-dong WANG 

机构地区：[1]College of Life Science, Liaocheng University [2]Agricultural Science Institute of Liaocheng

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2017年第8期674-684,共11页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the Natural Science Foundation of Shandong Province of China(No.ZR2015CL002);the Doctoral Research Startup Foundation of Liaocheng University(No.31805),China

摘　　要：An ultrasonic-assisted technique was employed to extract crude polysaccharide from Tricholoma matsutake fruiting bodies. Single-factor tests and orthogonal experimental design(L_9(3~3)) were used to obtain the optimal extraction conditions. Results showed that the optimal parameters were as follows: ultrasonic temperature, 40 °C; ultrasonic time, 50 min; water to raw material ratio, 25 ml/g; ultrasonic frequency, 45 k Hz; and ultrasonic power, 100 W. Three novel T. matsutake polysaccharide(TMP) fractions(TMP30, TMP60, and TMP80) were isolated and purified from TMP by stepwise alcohol precipitation. Their preliminary structural features were determined by highperformance anion-exchange chromatography with pulsed-amperometric detection(HPAEC-PAD) and Fourier transform infrared spectrophotometer(FT-IR) analyses. Furthermore, their in vitro antioxidant activity was investigated in terms of a reducing power assay and the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl(DPPH) and hydroxyl radicals. The order of the various fractions based on their antioxidant activity was TMP80>TMP>TMP60>TMP30. These findings suggested that novel polysaccharide fractions from T. matsutake, especially TMP80, could be promising active macromolecules for biomedical use.An ultrasonic-assisted technique was employed to extract crude polysaccharide from Tricholomamatsutake fruiting bodies. Single-factor tests and orthogonal experimental design （L9（33）） were used to obtain theoptimal extraction conditions. Results showed that the optimal parameters were as follows： ultrasonic temperature,40℃; ultrasonic time, 50 min; water to raw material ratio, 25 ml/g; ultrasonic frequency, 45 kHz; and ultrasonic power,100 W. Three novel T. matsutake polysacchadde （TMP） fractions （TMP30, TMP60, and TMP80） were isolated andpurified from TMP by stepwise alcohol precipitation. Their preliminary structural features were determined by high-performance anion-exchange chromatography with pulsed-amperometric detection （HPAEC-PAD） and Fouriertransform infrared spectrophotometer （FT-IR） analyses. Furthermore, their in vitro antioxidant activity was investigatedin terms of a reducing power assay and the scavenging rates of 2,2-diphenyl-l-picrylhydrazyl （DPPH） and hydroxylradicals. The order of the various fractions based on their antioxidant activity was TMP80〉TMP〉TMP60〉TMP30.These findings suggested that novel polysaccharide fractions from T. matsutake, especially TMP80, could be prom-ising active macromolecules for biomedical use.

关 键 词：TRICHOLOMA matsutake POLYSACCHARIDE Orthogonal test design Ultrasound-assisted extraction Mono-saccharide composition Antioxidant activity 

分 类 号：R284.2[医药卫生—中药学]