基于硫化镍的C-反应蛋白免疫传感器的研制  

Preparation of C-reactive Protein Immunousensor Based on Nickel Sulfide Material

作　　者：张坤蕾 李瑶[1] 白茹燕 张茜[1] 李德蕾 胡蓉[1] 杨云慧[1] 

机构地区：[1]云南师范大学化学化工学院,云南昆明650500

出　　处：《分析科学学报》2017年第4期483-487,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21465026;21165023)

摘　　要：本文合成了负载金纳米颗粒(Au NPs)的NiS纳米材料,通过壳聚糖(CHIT)将其固定在玻碳电极表面作为电化学生物传感器的固定基质。将C-反应蛋白(CRP)抗体固定到修饰过的玻碳电极表面,利用二茂铁甲酸标记CRP抗体,构建夹心型CRP生物传感器。采用差分脉冲伏安法(DPV)检测标记物二茂铁甲酸在0.3V左右的特征峰信号,该电流与培育的CRP抗原量成正比,从而实现对CRP的定量检测。传感器检测CRP的线性范围为0.01~500ng/mL,线性相关系数为0.9939,检测限为3.3pg/mL。In this article,nickel sulfide was synthesized and used to load gold nanoparticles（Au NPS）and modified on the surface of glassy carbon electrode by chitosan as immobilized matrix to fix C-reactive protein antibody（anti-CRP）.Ferroennecarboxylic acid was labeled on the anti-CRP to fabricate sandwich type of CRP immunosensor.Differential pulse voltammetry was used to detect a reduction signal peak of ferrocenecarboxylic acid at 0.3V which is proportional to the concentration of CRP antigen.Under the optimal experimental conditions,the linear range was 0.01-500ng/mL with a detection limit of 3.3pg/mL.This immunosensor provides a method for effective and sensitive detection of CRP.

关 键 词：C-反应蛋白 NIS 二茂铁甲酸 电化学 免疫传感器 

分 类 号：O657.1[理学—分析化学]