β-环糊精衍生物/Cu_2O毛细管电色谱整体柱的制备及其应用  

Preparation and Application of Capillary Electrochromatography Monolithic Column of β-CDs/Cu_2O

作　　者：李英杰[1] 尹丽娟[1] 高立娣[1] 吕仁江[1] 刘树仁[1] 王宁[1] 崔丽燕[1] 

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006

出　　处：《分析科学学报》2017年第4期507-511,共5页Journal of Analytical Science

基　　金：黑龙江省自然科学基金(No.B2016009)

摘　　要：采用一步键合法制备了烯丙胺-β-环糊精/Cu_2O(Ally-β-CD/Cu_2O)毛细管电色谱整体柱,并考察了制备整体柱的主要影响因素。通过扫描电子显微镜(SEM)、X-射线光电子能谱(XPS)、X-射线衍射(XRD)对整体柱柱内固定相进行表征。以硫脲为中性标记物测定了柱效,柱效达到47 658 N/m。对D,L-组氨酸对映体的分离度RS达到3.82,整体柱在连续使用72h或间歇使用2个月后仍具有良好的分离能力,表明整体柱具有良好的重现性和稳定性,同时具备较好的手性拆分能力。运用该整体柱对盐酸克伦特罗对映体进行了拆分,达到基线分离,分离度达到2.89。In this paper,the capillary electrochromatography monolithic column of β-CDs/Cu_2O was prepared by one-step bonding method.The main factors in preparing monolithic column were investigated.The stationary phase of monolith column was characterized by scanning electron microscopy（SEM）,X-ray photoelectron spectroscopy（XPS）,X-ray diffraction（XRD）.The column efficiency of monolith column was up to 4 7658 N/m with thiourea as neutral markers.The separation of D,L-histidine enantiomers reached 3.82.These results show that the monolith column has good reproducibility and stability,along with a better chiral separation ability.Baseline separation was achieved for the enantiomers of clenbuterol hydrochloride,with the resolution of 2.89.

关 键 词：β-环糊精衍生物/Cu2O 整体柱 盐酸克伦特罗 手性拆分 

分 类 号：O657.8[理学—分析化学]