P-15肽促进间充质干细胞向软骨细胞分化的实验研究  

P15 peptide enhances Mesenchymal stem cells chondrocyte differentiation and formation:a experimental study in vitro

作　　者：张郡[1] 杨利丽[1] 刘钦毅[1] 孟宪荣[1] 刘睿[1] 王瑞强[1] 田海清[1] 万腾[1] 

机构地区：[1]吉林大学第二医院,吉林长春130041

出　　处：《中国实验诊断学》2017年第8期1425-1427,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的本研究探讨P-15肽能否有效激活、促进软骨细胞向增殖分化。方法在不同的培养基培养鼠间充质干细胞(mesenchymal stem cells,MSCs),培养细胞采用阿辛蓝染色和碱性磷酸酶染色,并行碱性磷酸酶定量;免疫组织化学及蛋白免疫印迹检测软骨形成特异性标记。结果 (1)与对照组相比,P-15肽组呈现大量的碱性磷酸酶阳性细胞,蛋白聚糖的合成明显增加;(2)免疫组织化学检测提示P-15肽组collagen X(COLX)、Runt-related transcription factor 2(RUNX2)、matrix metallopeptidase 13(MMP13)明显高于对照组;(3)蛋白免疫印迹检测提示P-15肽组在RUNX2、MMP-13、Vascular endothelial growth factor(VEGF)、COLX几个因子表达明显升高(P<0.05)。结论 P-15肽能有效激活、促进间充质干细胞增殖和向软骨细胞分化。Objective The purpose of the experiment is to clarify whether SyntheticpeptideP15 can promotes chondrogenic differentiation and formation in vitro.Methods Mesenchymal stem cells（MSCs）was cultured in differentiation media.Alkalinephosphatase and alcian blue staining was performed.Chondrogenic markers were measured by Western blot and fluorescent immunohistochemistry.Results Compared with the uncoated controlplates the P15 peptide cultured cells showed intense alkalinephosphatase（P〈0.05）and alcian blue staining.Immunofluorescent staining indicatedthat there was a significant increase in the levels ofrunt-relatedtranscription factor-2（RUNX2）,matrix metalloproteinase-13（MMP13）,and collagen X（COLX）proteins.Increased expression of RUNX2,COLX,MMP13 and vascular endothelial growth factor（VEGF）was also confirmed byWestern blot analysis（P〈0.05）.Conclusion P15 peptide enhances chondrocyte differentiation and formation in vitro.

关 键 词：P-15肽 软骨细胞 软骨内骨化 增殖 分化 

分 类 号：R683[医药卫生—骨科学]