毛茛叶报春高分化的谱系地理结构  

Highly differentiated phylogeographic structure of Primula ranunculoides

作　　者：宋丽雅[1] 李永权[1] 章伟[1] 邵剑文[1,2] 

机构地区：[1]安徽师范大学生命科学学院,安徽芜湖241000 [2]安徽省重要生物资源保护与利用研究重点实验室,安徽芜湖241000

出　　处：《植物科学学报》2017年第4期503-512,共10页Plant Science Journal

基　　金：国家自然科学基金项目(31570336);生物环境与生态安全安徽省高校重点实验室项目~~

摘　　要：毛茛叶报春(Primula ranunculoides Chen)是我国特有的珍稀濒危花卉,其野生种质资源已十分稀少。为了揭示其谱系地理结构,本文对11个居群114个个体的叶绿体trn L-F和trn S-G基因片段进行了测序分析。结果共检测到10个单倍型,除YJPA和YJPB两居群共享一个单倍型(H9)外,其余各居群均仅有一个特有单倍型,居群内不存在单倍型多样性。毛茛叶报春具高分化的谱系地理结构,居群间的总平均遗传距离达0.015,单倍型间的平均突变次数高达31.2步,两者均显著高于同属的近缘种。其种子散布能力弱、生境片断化和地理隔离明显及两年生的生活史可能是导致其高分化谱系地理结构产生的主要原因。Samova、Structure以及TCS分析均支持将毛茛叶报春11个居群的遗传结构分为6组,它们与地形(山脉)关系密切对应,彼此间分化明显,因此建议不同山体的居群应视为不同的保护单元进行遗传管理。Primula ranunculoides Chen is a rare and endangered species endemic to China.To reveal its phylogeographic patterns,the trnL-F and trnS-G plastid DNA of 114 individuals sampled from 11 natural populations were sequenced and analyzed.In total,10 haplotypes were identified,and populations were fixed with only one unique haplotype,except for populations YJPA and YJPB,which shared a haplotype （H9）.Primula ranunculoides has a highly differentiated and significant phylogeographic structure,with an average genetic distance among populations of 0.015 and average mutation between haplotypes of 31.2 steps,significantly higher than those of related species within the genus.The high differentiation between the populations and haplotypes of this species might be explained by its limited seed dispersal ability,habitat fragmentation,isolation of geographical distance,and biennial life form.Samova,Structure,and TCS analyses unanimously supported the clustering of the 11 sampled populations into six groups,which were closely correlated with topographical features and highly differentiated among each other.Accordingly,populations from different mountains should be considered as different genetic management units for conservation purposes.

关 键 词：毛茛叶报春 谱系地理结构 叶绿体DNA 保护单元 

分 类 号：Q948[生物学—植物学]