铜胁迫对不同抗性种群海州香薷酸性转化酶基因启动子甲基化的影响  

DNA methylation patterns of acid invertase gene promoters from Cu-tolerant and non-tolerant populations of Elsholtzia haichowensis under copper stress

作　　者：何宇宁[1] 徐仲瑞[1] 熊治廷[1] 

机构地区：[1]武汉大学资源与环境科学学院,武汉430079

出　　处：《植物科学学报》2017年第4期574-582,共9页Plant Science Journal

基　　金：国家自然科学基金项目(21477093)~~

摘　　要：采用亚硫酸氢盐测序技术,检测了Cu胁迫对海州香薷(Elsholtzia haichowensis Sun)Cu抗性和非抗性种群酸性转化酶基因启动子甲基化的影响。结果表明,海州香薷液泡转化酶基因(v INV)和细胞壁转化酶基因(cw INV)的启动子在CG位点分别表现出超甲基化和低甲基化的现象。酸性转化酶基因启动子CHG和CHH位点的甲基化状态受Cu胁迫影响较大。Cu胁迫下,v INV和cw INV启动子分别有1个CHG和6个CHH位点的甲基化状态在Cu抗性种群和非抗性种群之间表现出较大差异。抗Cu种群中这些甲基化差异位点对Cu胁迫不敏感,但在非抗性种群中这些位点的甲基化水平在Cu胁迫后出现大幅上升或下降。有些甲基化差异位点位于或者临近预测的启动子顺式作用元件区域,可能参与Cu胁迫下酸性转化酶基因的表达调控。In the present study,bisulfite sequencing （BS-seq） was performed to detect alterations in DNA methylation of acid invertase gene promoters in Cu-tolerant （MP） and non-tolerant populations （NMP） of metallophyte Elsholtzia haichowensis under Cu treatment.We found that the vacuolar invertase gene （vINV） promoter of E.haichowensis was generally methylated in the CG site,whereas the cell wall invertase gene （cwINV） promoter displayed hypomethylation at the CG site.Methylation changes at a special CHG site of the vINV promoter and six CHH sites of the cwINV promoter induced by Cu stress were observed between the two populations.In addition,CG methylation was relatively conserved in the vINV and cwINV promoters under Cu stress,whereas methylation at the CHG and CHH sites was more prone to perturbation.Under Cu stress,methylation of these individual sites remained stable in the MP,but declined or increased drastically in the NMP.This implied that MP might have evolved modulating mechanisms due to long-term Cu stress,including changes in methylation patterns in specific DNA sequences and cytosine sites.

关 键 词：酸性转化酶 启动子 DNA甲基化 CU胁迫 海州香薷 

分 类 号：Q943.2[生物学—植物学]