阿霉素诱导的扩张型心肌病大鼠心律失常与内质网应激相关性研究  

Correlation study of arrhythmia and endoplasmic reticulum stres in dilated cardiomyopathy rats induced by adriamycin

作　　者：贺智慧[1] 刘灏[2] 王羽[1] 宣丽颖[1] 王春贵[1] 魏成喜[1] 赵明[2] 

机构地区：[1]内蒙古民族大学医学院,内蒙古通辽028000 [2]内蒙古民族大学附属医院

出　　处：《临床心血管病杂志》2017年第8期798-801,共4页Journal of Clinical Cardiology

基　　金：国家自然科学基金项目(No:81360587);内蒙古民族大学科学研究项目(No:NMDYB1733)

摘　　要：目的:研究阿霉素诱导的扩张型心肌病大鼠心律失常与内质网应激的关系。方法:30只Wister雄性大鼠分为对照组、阿霉素模型组(模型组),每组15只,对照组腹腔注射0.9%Nacl(10ml/kg),共3次(每周1次);模型组腹腔注射阿霉素2mg/kg,共注射3次(每周1次)。7周末2组大鼠行心电图检测心律失常发生率及心脏彩超检测左室舒张末径及左心室射血分数,2组大鼠处死后取左心室组织行苏木精-伊红、masson、VG染色,观察心肌病理改变,采用real time PCR及Western blotting技术检测2组大鼠心肌细胞内质网应激伴侣蛋白GRP78,GRP94表达。结果:与对照组大鼠比较,模型组大鼠心律失常发生率增多(P<0.05),心肌细胞内质网应激伴侣蛋白GRP78,GRP94表达明显增多(P<0.01)。结论:阿霉素诱导扩张型心肌病大鼠心律失常发生可能与心肌细胞内质网应激相关。Objective：To investigate the correlation of arrhythmia and endoplasmic reticulum stress in dilated cardiomyopathy rats induced by adriamycin.Method：Thirty male wister rats were randomly divided into control group and ADR group.The rats of ADR group were intraperitoneally injected 2mg/kg/week ADR for 3weeks,while the rats of control group were intraperitoneally injected 10ml/kg in one week 0.9% Nacl for 3weeks.Arrhythmia was detected by electrocardiogram,and left ventricular end diastolic diameter and left ventricular ejection fraction were analyzed by echocardiography after 7weeks.Rats in the two groups were sacrificed and the left ventricle stained by HE,Masson and VG staining.The expression of GRP78 and GRP94were detected by real-time PCR and western blot.Result：（1）Compared with control group,the incidence of arrhythmia in ADR group was increased（P0.05）.（2）Compared with control group,the expressions GRP78 and GRP94were enhanced in ADR group（P0.01）.Conclusion：the arrhythmia in dilated cardiomyopathy rats induced by adriamycin may related with endoplasmic reticulum stress of myocardium.

关 键 词：扩张型心肌病 心律失常 内质网应激 

分 类 号：R542.2[医药卫生—心血管疾病]