锰中毒大鼠海马锌含量与锌转运蛋白的关系  

Relationship between concentrations of zinc and zinc transporter in hippocampus of manganism rat

作　　者：粟立羽 丁宏伟[1] 胡斌丽[1] 李红[1] 李岩[1] 

机构地区：[1]遵义医学院公共卫生学院卫生毒理学教研室,贵州遵义563000

出　　处：《环境与职业医学》2017年第8期718-721,共4页Journal of Environmental and Occupational Medicine

基　　金：贵州省科学技术厅国际科技合作项目(编号:黔科合外G字[2014]7012号)

摘　　要：[目的]探讨锰中毒大鼠海马区锰和锌含量变化及与锌转运蛋白3(ZnT3)之间的关系。[方法]将40只雄性SD大鼠随机分成4组,每组10只,即生理盐水对照组和染锰低剂量、中剂量、高剂量组。分别给予腹腔注射生理盐水及氯化锰溶液3、6、12 mg/(kg·d)连续注射8周,锰中毒造模成功后取大鼠海马组织,做HE染色病理切片观察其病理改变,石墨炉原子吸收仪测定海马锰、锌含量变化,Western blot法检测大鼠海马区ZnT3含量,实时荧光定量PCR法测定大鼠海马区ZnT3 mRNA的表达。[结果]染毒后大鼠海马神经细胞均出现不同程度嗜酸性变,胞浆凝聚、结构不清,且出现细胞皱缩和嗜酸性小体。与对照组及低剂量组比较,高、中剂量组大鼠海马组织中锰及锌含量均明显增高(P<0.05);而高剂量组锌含量也高于中剂量组(P<0.01)。高、中、低剂量组大鼠海马组织中ZnT3含量分别为1.01±0.03、0.80±0.02、0.65±0.13,均高于对照组(0.51±0.06)(P<0.05)。高、中剂量组ZnT3基因相对表达分别为3.40±0.36、2.88±0.69,均高于对照组及低剂量组(1.47±0.60)(P<0.001)。[结论]锰中毒可破坏大鼠海马神经元细胞结构,且海马组织中锌含量与ZnT3同步增高。[ Methods ] Forty male SD rats were randomly divided into four groups with 10 rats in each group： high-dose group, middle- dose group, low-dose group, and control group. The four groups were given intraperitoueal injection of 3, 6, and 12 mg/（kg·d） MnCI2 solutions and normal saline （NS）, respectively, for eight weeks. Animals in each group were sacrificed and hippocampus tissue samples were removed for determination. We observed the pathologic changes of hippocampi of the four groups by HE staining under microscope, detected Mn^2＋ and Zn^2＋ in hippocampus tissue by graphite furnace atomic absorption spectrometry, determined the concentration of ZnT3 protein by Western blot, and examined the expression of ZnT3 mRNA by real-time fluorescent quantitative PCR in rat hippocampus. [ Results ] All groups exposed to manganese were observed changes in rat hippocampal neurons with eosinophilic degeneration, condensed cytoplasm, unclear cell composition, cell shrinkage, and eosinophilic body. Compared with the control group and the low- dose group, the concentrations of manganese and zinc were significantly higher in the high-dose group and the middle-dose group （P 〈 0.05）, and the concentration in the high-dose group was higher than that in the middle-dose group （P 〈 0.01）. The expression levels of ZnT3 protein were 1.01 ± 0.03, 0.80 ± 0.02, 0.65 ± 0.13, respectively, in the high-dose, middle-dose, and low-dose groups,higher than that of the control group （0.51 ± 0.06） （P〈0.05）. The expression levels ofZnT3 mRNA were 3.40 ± 0.36 and 2.88 ± 0.69, respectively, in the high-dose and middle-dose groups, higher than those in the control group and the low-dose group （1.47 ± 0.60） （P〈0.001）. [ Conclusion ] Manganese can damage the structure of rat hippocampus neurons, and the concentration of zinc in hippocampus is elevated with the increase of ZnT3 in hippocampus.

关 键 词：锰中毒 海马 锌转运蛋白 大鼠 锌 

分 类 号：R114[医药卫生—卫生毒理学]