正交实验法优选发酵蜈蚣粉中水溶性总蛋白提取工艺及其抗肿瘤效果  

Optimization of Extraction Process for Total Water Soluble Proteins in Fermented Centipede Powders by Orthogonal Test

作　　者：史磊[1] 刘春雨[1] 张盼盼[1] 王彦多[1] 王颖[1] 王集会[2] 

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东中医药大学实验中心,山东济南250355

出　　处：《山东中医杂志》2017年第8期710-713,共4页Shandong Journal of Traditional Chinese Medicine

基　　金：山东中医药大学大学生研究训练(SRT)计划资助项目(校编号:2015308;国家编号:201610441069)

摘　　要：目的:优选中药发酵蜈蚣粉中水溶性总蛋白的最佳提取工艺。方法:在对中药发酵蜈蚣粉中总蛋白提取条件进行单因素考察的基础上,采用L_9(3~4)正交实验设计法,以发酵蜈蚣粉水溶性总蛋白含量为指标,考察提取时间、pH值、料液比三个因素对提取物中的水溶性总蛋白含量的影响,优选出最佳提取工艺。结果:中药发酵蜈蚣粉水溶性总蛋白的最佳提取工艺为:向发酵蜈蚣粉中加入20倍量的pH值为9的蒸馏水超声提取150 min。在最佳工艺下提取发酵蜈蚣粉中总蛋白对乳腺癌MCF-7细胞具有很好的抑制效果。结论:实验中优选的发酵蜈蚣粉中总蛋白提取工艺简便、可行,能准确反映总蛋白含量和一定的抗肿瘤效果。Objective：To optimize extraction process for total water soluble proteins in fermented centipede powders. Method ：With the water soluble proteins as the index,the orthogonal experiment design method of L9 （34） was used to evaluate the influence by extraction time,pH value,and material-liquid proportions on content of the water soluble total proteins of centipede powder,based on the study for single factors ,and the optimized extraction process was determined. Results：The optimized extraction process for water-soluble total protein from the fermented centipede powder was as follows：adding 20-fold distilled water with pH value of 9 and under ultrasonic extraction for 150 min;the water soluble extractions of the fermented centipede powder had a good inhibitory effect on mammary gland MCF-7 ceils. Conclusion：In this experiment,the optimized extraction process of total protein from fermented centipede powder was convenient and feasible,and can accurately reflect the total protein content with certain anti-tumor effect.

关 键 词：发酵蜈蚣粉 总蛋白 MTT法 乳腺癌MCF-7细胞株 肺腺癌A549细胞株 细胞抑制率 

分 类 号：R284.2[医药卫生—中药学]