Overexpression of GhPFN2 enhances protection against Verticillium dahliae invasion in cotton  

作　　者：Wenyan Wang Yongduo Sun Libo Han Lei Su Ouixian Xia Haiyun Wang 

机构地区：[1]Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [2]State Key Laboratory of Plant Genomics,Beijing 100101,China [3]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Science China(Life Sciences)》2017年第8期861-867,共7页中国科学（生命科学英文版）

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB11040600);the National Natural Science Foundation of China(31671278);the State Key Laboratory of Plant Genomics of China(2015B0129-02)

摘　　要：Growing evidence indicates that actin cytoskeleton is involved in plant innate immune responses, but the functional mechanism remains largely unknown. Here, we investigated the behavior of a cotton profilin gene （GhPFN2） in response to Verticillium dahliae invasion, and evaluated its contribution to plant defense against this soil-borne fungal pathogen. GhPFN2 expression was up-regulated when cotton root was inoculated with V. dahliae, and the actin architecture was reorganized in the infected root cells, with a clear increase in the density of filamentous actin and the extent of actin btmdling. Compared to the wild type, GhPFN2-overexpressing cotton plants showed enhanced protection against V. dahliae infection and the actin cytoskeleton organization in root epidermal cells was clearly altered, which phenocopied that of the wild-type （WT） root cells challenged with V. dahliae. These results provide a solid line of evidence important for defense against V. dahliae infection. showing that actin cytoskeleton reorganization involving GhPFN2 isGrowing evidence indicates that actin cytoskeleton is involved in plant innate immune responses,but the functional mechanism remains largely unknown.Here,we investigated the behavior of a cotton profilin gene(GhPFN2) in response to Verticillium dahliae invasion,and evaluated its contribution to plant defense against this soil-borne fungal pathogen.GhPFN2 expression was up-regulated when cotton root was inoculated with V.dahliae,and the actin architecture was reorganized in the infected root cells,with a clear increase in the density of filamentous actin and the extent of actin bundling.Compared to the wild type,GhPFN2-overexpressing cotton plants showed enhanced protection against V.dahliae infection and the actin cytoskeleton organization in root epidermal cells was clearly altered,which phenocopied that of the wild-type(WT) root cells challenged with V.dahliae.These results provide a solid line of evidence showing that actin cytoskeleton reorganization involving GhPFN2 is important for defense against V.dahliae infection.

关 键 词：actin cytoskeleton PROFILIN Verticillium dahliae COTTON 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]