不同水平肌醇对獭兔皮肤中β-连环蛋白和激素敏感脂酶表达的影响  

Effects of Different Inositol Levels on β-Catenin and Hormone-Sensitive Triglayceride Lipase Expression in Skin of Rex Rabbits

作　　者：李敏[1] 崔亚利[1] 王丙雷 陈宝江[2] 

机构地区：[1]河北农业大学动物医学院,保定071000 [2]河北农业大学动物科技学院,保定071000

出　　处：《动物营养学报》2017年第8期2743-2750,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家兔产业技术体系(CARS-44-B-3);吉林海资生物技术有限公司横向课题

摘　　要：本试验旨在研究饲粮中添加不同水平的肌醇对獭兔皮肤中β-连环蛋白(β-catenin)和激素敏感脂酶(HSL)表达的影响。选取(40±1)日龄的同期断奶獭兔120只(公母各占1/2),随机分成4组,每组30只。在4组獭兔的饲粮中分别添加0、25、50、75 mg/kg的肌醇,试验期为3个月。在试验的第30天(2月龄)、第60天(3月龄)和第90天(4月龄),分别取腹部、背中部、臀部皮肤,采用免疫组织化学和蛋白质印迹(Western blot)法对β-catenin和HSL的蛋白质表达和定位进行检测。结果表明:β-catenin在毛囊中广泛表达,在毛根鞘细胞和毛乳头均有棕黄色阳性反应细胞。HSL在毛根鞘细胞,尤其是在内根鞘细胞内,呈现非常明显的棕黑色强阳性表达。饲粮中添加50 mg/kg肌醇可以极显著增加2~4月龄獭兔背中部皮肤毛囊中β-catenin和HSL阳性表达细胞的平均灰度值(P<0.01),极显著增加4月龄獭兔背中部、腹部和臀部皮肤毛囊中β-catenin和HSL阳性表达细胞的平均灰度值(P<0.01)。结果提示,肌醇能够通过上调4月龄獭兔毛囊中β-catenin和HSL的表达来促进毛囊的发育,在本试验中,饲粮肌醇水平达到50 mg/kg时效果最佳。The aim of the present study was to investigate the effects of different inositol levels in diets on the expression ofβ-catenin and hormone-sensitive triglayceride lipase （ HSL） in skin of Rex rabbits. A total of 120 （40＆#177;1）-day-old Rex rabbits （half male and half female） were randomly divided into 4 groups with 30 rabbits per group. Rex rabbits in 4 groups were fed diets with different levels of inositol （ 0, 25, 50 and 75 mg/kg, respectively） , and the experiment lasted for 3 months. The skin of abdomen, dorsocentral region, hip were collected on days 30 （ 2-month-old） , 60 （ 3-month-old） , 90 （ 4-month-old） , respectively, and the protein ex-pression and location of β-catenin and HSL were studied by immunohistochemistry and Western blot methods. The results showed that the β-catenin was widely expressed in hair follicle, and the hair root sheath cells and hair papilla all had brown positive reaction cells. HSL was showed a very obvious dark brown strong positive expression in hair root sheath cells, especially in inner root sheath cells. The addition of 50 mg/kg inositol could extremely significantly increase the mean gray value ofβ-catenin and HSL positive cells in the hair folli-cles of the dorsocentral region skin of 2- to 4-month-old Rex rabbits （P〈0.01）, extremely significantly in-crease the mean gray value of β-catenin and HSL positive cells in the hair follicles of the dorsocentral region, abdomen and hip skin of 4-month-old Rex rabbits （ P〈0.01） . The results suggest that inositol can promote the growth of hair follicle by increasing the expresstion ofβ-catenin and HSL of 4-month-old Rex rabbits, and the effect is the best when dietary inositol level is 50 mg/kg in this experiment.

关 键 词：肌醇 獭兔 毛囊 Β-连环蛋白 激素敏感脂酶 

分 类 号：S816.7[农业科学—饲料科学]