玉米赤霉烯酮对断奶小母猪生产性能、血清抗氧化功能和免疫功能的影响  

Effects of Zearalenone on Production Performance,Serum Antioxidant Capacity and Immune Function of Weaning Gilts

作　　者：杨立杰[1] 王淑静[1] 杨维仁[1] 黄丽波[1] 刘法孝[1] 姜淑贞[1] 杨在宾[1] 

机构地区：[1]山东农业大学动物科技学院,泰安271018

出　　处：《动物营养学报》2017年第8期2843-2850,共8页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家自然科学基金(31572441);山东省现代农业产业技术体系生猪创新团队建设项目(SDAIT-08-04)

摘　　要：为了研究不同水平玉米赤霉烯酮(zearalenone,ZEA)污染饲粮对断奶小母猪生产性能、血清抗氧化功能、血清抗体水平及外周血淋巴细胞增殖率的影响。将40头健康三元杂交(杜×长×大)断奶小母猪按日龄[(35±1)日龄]和平均体重[(14.01±0.86)kg]分为4组,对照组饲喂基础饲粮(ZEA水平的测定值为0 mg/kg),试验组在基础饲粮中分别添加0.5、1.0及1.5 mg/kg ZEA[ZEA水平的测定值分别为(0.52±0.07)mg/kg、(1.04±0.03)mg/kg和(1.51±0.13)mg/kg]。预试期10 d,正试期35 d。结果表明:饲粮ZEA对断奶小母猪平均日采食量、平均日增重和料重比没有显著影响(P>0.05)。与对照组相比,ZEA显著降低了血清谷胱甘肽过氧化物酶(GSH-Px,0.5、1.0和1.5 mg/kg ZEA)活性、猪瘟(1.0和1.5 mg/kg ZEA)和伪狂犬病病毒抗体水平(1.5 mg/kg ZEA)以及外周血淋巴细胞增殖率(1.0和1.5 mg/kg ZEA)(P<0.05),而显著升高了血清丙二醛(MDA,0.5、1.0和1.5 mg/kg ZEA)含量(P<0.05)。随着饲粮中ZEA水平的升高,断奶小母猪的料重比呈一次线性降低趋势(P=0.075),血清GSH-Px、超氧化物歧化酶(SOD)活性,血清病毒(猪瘟、伪狂犬病和高致病性猪蓝耳病病毒)抗体水平和外周血淋巴细胞增殖率均呈一次线性降低(P<0.05),而血清MDA含量则呈一次线性升高(P<0.05)。由此可见,饲粮中0.5 mg/kg的ZEA足以诱导小母猪的氧化应激反应,1.0 mg/kg的ZEA能够显著降低断奶小母猪的特异性体液免疫和细胞免疫功能。The aim of the present study was to investigate the effects of diet polluted by different levels of zearalenone （ ZEA） on performance, serum antioxidant capacity, serum antibody levels and lymphocyte prolif-eration rate of weaning gilts. Forty healthy weaning piglets （ Duroc ＆#215; Landrace ＆#215; Large white） were allocated into 4 groups according to age [（35±1） days of age] and average body weight [（14.01±0.86） kg]. Piglets were fed a basal diet supplemented with 0 （ Control） , 0. 5, 1. 0 and 1. 5 mg/kg ZEA, respectively, and the measured values of ZEA were 0 mg/kg, （ 0. 52 ± 0. 07 ） mg/kg, （ 1. 04 ± 0. 03 ） mg/kg and （ 1. 51 ± 0.13） mg/kg, respectively. The adaptation period lasted for 10 days and the trial period lasted for 35 days. The results showed as follows： average daily feed intake, average daily gain and feed to gain ratio （ F/G） of weaning piglets were not significantly affected by dietary ZEA （ P〉0.05） . Compared with control group, ser-um glutathione peroxidase （ GSH-Px） activity in 0.5, 1.0 and 1.5 mg/kg ZEA groups, antibody levels of clas-sical swine fever virus in 1.0 and 1.5 mg/kg ZEA groups and herpes virus in 1.5 mg/kg ZEA group, and lym-phocyte proliferation rate in peripheral blood in 1. 0 and 1. 5 mg/kg ZEA groups were significantly decreased （P〈0.05）, however, serum malondialdehyde （MDA） content in 0.5, 1.0 and 1.5 mg/kg ZEA groups was significantly increased （ P〈0.05） . With the increase of ZEA level in diet for weaning gilts, feed to gain ratio showed a linear decreasing trend （ P=0.075） , serum activities of GSH-Px, superoxide dismutase （ SOD） , se-rum antibody levels of viruses （ classical swine fever virus, herpes virus and porcine reproductive and respirato-ry syndrome virus） and peripheral blood lymphocyte proliferation rate in peripheral blood showed linear decrea-ses （ P〈0.05） , and serum MDA showed a linear increase （ P〈0.05） . Thus, ZEA at 0.5 mg/kg is sufficient to induce oxidative

关 键 词：玉米赤霉烯酮 断奶小母猪 生产性能 谷胱甘肽过氧化物酶 丙二醛 抗体水平 淋巴细胞增殖率 

分 类 号：S816[农业科学—饲料科学] S828[农业科学—畜牧学]