维生素E对日本沼虾生长性能、抗氧化性能及抗氨氮胁迫能力的影响  

Effects of Dietary Vitamin E on Growth Performance,Antioxidant Ability and Resistance to Ammonia Nitrogen Stress of Macrobrachium nipponense

作　　者：孔有琴[1] 丁志丽[1] 张易祥[1] 罗娜[1,2] 叶金云[1] 

机构地区：[1]浙江省水生生物资源养护与开发技术研究重点实验室,中国水产科学研究院水生动物繁育与营养重点实验室,湖州师范学院生命科学学院,湖州313000 [2]大连海洋大学水产与生命科学学院,大连116000

出　　处：《动物营养学报》2017年第8期2893-2905,共13页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：浙江省重大科技专项计划项目(2014C02011);浙江省自然科学基金项目(LY16C190006);国家自然科学基金项目(31402308);湖州市自然科学资金项目(2015YZ06);浙江省重点研发计划项目(2015C03018)

摘　　要：本试验旨在研究饲料中维生素E水平对日本沼虾(Macrobrachium nipponense)幼虾生长性能、抗氧化性能和抗氨氮胁迫能力的影响。选择健壮、平均初始体重为(0.119±0.004)g的900只日本沼虾幼虾,随机分为6个组,每组3个重复,每个重复50只。以维生素E乙酯为添加形式,配制6组维生素E实际含量分别为18.31、37.94、66.07、120.25、212.68和388.96 mg/kg的半纯化饲料(分别记为VE1、VE2、VE3、VE4、VE5和VE6组),饲喂8周,随后进行24 h氨氮胁迫试验。结果显示:1)各组日本沼虾成活率(SR)无显著差异(P>0.05);随饲料维生素E水平的增加,日本沼虾增重率(WGR)呈先增加后下降的变化趋势,VE4组最高,且显著高于VE1组(P<0.05);饲料系数(FCR)变化趋势则与WGR相反,VE4组最低,且显著低于VE1组(P<0.05)。2)氨氮胁迫前,各组日本沼虾肝胰腺中丙二醛(MDA)含量随饲料维生素E水平的增加呈先下降后上升的变化趋势,在VE3组达到最低,且显著低于VE1、VE5和VE6组(P<0.05);各组日本沼虾肝胰腺总超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活力和总抗氧化力(T-AOC)随饲料维生素E水平的增加呈先上升后下降的变化趋势。VE1和VE6组日本沼虾硫氧还蛋白(Trx)mRNA表达量较高,硫氧还蛋白还原酶(TrxR)mRNA表达量则较低。VE3组热休克蛋白60(HSP60)mRNA表达量显著低于其他各组(P<0.05),VE1组热休克同源蛋白70(HSC70)mRNA表达量显著低于其他各组(P<0.05),VE1和VE2组热休克蛋白90(HSP90)mRNA表达显著高于其他各组(P<0.05)。3)氨氮胁迫后,各组日本沼虾肝胰腺SOD、CAT、GSH-Px活力,MDA含量,Trx、TrxR和HSC70 mRNA表达量的变化趋势与胁迫前相似,HSP60和HSP90 mRNA表达量变化与胁迫前相反。氨氮胁迫可降低日本沼虾肝胰腺GSH-Px活力、T-AOC及Trx、TrxR mRNA表达量,提高MDA含量及VE4、VE5和VE6组SOD活力。由此可见,饲料中添加120.25 mg/kg维生素E对日本沼虾的生长具有积�The present experiment was conducted to evaluate the effects of dietary vitamin E level on growth performance, antioxidant ability and resistance to ammonia nitrogen stress of juvenile Macrobrachium nippon-ense. A total of 900 healthy juvenile Macrobrachium nipponense with the body weight of （0.119±0.004） g were selected and randomly divided into 6 groups with 3 replicates per group and 50 shrimps per replicate. Vita-min E acetate was added to basal semi-purified diet at six levels to make diets with the actual content of 18.31, 37.94, 66.07, 120.25, 212.68 and 388.96 mg /kg vitamin E （ denoted by VE1, VE2, VE3, VE4, VE5 and VE6 groups, respectively） , and feeding for 8 weeks. After the feeding experiment, the prawns were subjected to ammonia nitrogen stress for 24 h. The results showed as follows：1） the survival rate of Macrobrachium nip-ponense in each group had no significant difference （ P〉0.05） . The weight gain rate showed a trend of firstly increased and then decreased with the dietary vitamin E increasing, and prawns in VE4 group gained the high-est value and significantly higher than that in VE1 group （ P〈0.05） . The feed conversion rate had the opposite pattern from the weight gain rate, the lowest value were found in VE4 group and significantly lower than that in VE1 group （ P〈0.05） . 2） Before ammonia nitrogen stress, the maleic dialdehyde （ MDA） content in hepato-pancreas exhibited a trend of firstly decreased and then increased with dietary vitamin E increasing, the lowest value was found in VE3 group and significantly lower than that in VE1, VE5 and VE6 groups （P〈0.05）, whereas the activities of superoxide dismutase （ SOD ） , glutathione peroxidase （ GSH-Px ） , catalase （ CAT ） and total antioxidant competence （ T-AOC） in hepatopancreas exhibited a trend of firstly increased and then de-creased with dietary vitamin E increasing. VE1 and VE6 groups gained the higher thioredoxin （ Trx） mRNA expression and the lower thioredoxin reductase �

关 键 词：日本沼虾 维生素E 生长 抗氧化力 氨氮 

分 类 号：S966.12[农业科学—水产养殖]