小半夏汤对化疗大鼠胃肠黏膜屏障保护作用的研究  

Research on the protecting effect of Xiaobanxia Decoction on the gastrointestinal mucosal barrier of rats after chemotherapy

作　　者：张启龙[1] 李贵生[1] 杜静[1] 聂克[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中药药理与临床》2017年第3期5-7,共3页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(项目批准号:81373828;81673779);山东省高等学校科技计划项目(编号:J16LK07)

摘　　要：目的:观察小半夏汤对化疗大鼠胃肠黏膜屏障的影响,以研讨其对化疗所致胃肠道黏膜损伤(Gastrointestinal mucositis,GIM)的保护作用和机制。方法:雄性Wistar大鼠112只随机分为空白对照组、小半夏汤正常对照组、顺铂模型组、奥美拉唑组、小半夏汤0.8、1.6、3.2 g/kg剂量组。各组分别灌胃蒸馏水、小半夏汤0.8 g/kg、蒸馏水、奥美拉唑1.2 mg/kg、小半夏汤0.4 g/kg、0.8 g/kg、1.6 g/kg,一日两次。灌胃3天,除两个正常对照组腹腔注射生理盐水,其余各组腹腔注射6 mg/kg顺铂建立胃肠黏膜损伤模型。每24 h记录动物体质量和摄食量;造模后24、72 h每组分别取8只大鼠取血、胃窦和回肠,酶联免疫法检测血清中黏蛋白2(MUC2)、黏蛋白5ac(MUC5ac)和D-乳酸水平,免疫组化染色检测胃窦MUC5ac、回肠MUC2、胃肠黏膜细胞增殖核抗原Ki-67的表达。结果:与正常对照组比较,模型组大鼠血清MUC2、MUC5ac水平显著下降、胃肠黏膜Ki-67表达显著降低、回肠MUC2和胃窦MUC5ac表达显著下降;小半夏汤3.2 g/kg可以上调模型动物血清与胃肠组织中MUC2、MUC5ac的表达,提高胃肠黏膜Ki-67表达。结论:小半夏汤对胃肠黏膜损伤具有防治作用,其机制可能与促进黏膜杯状细胞分泌黏蛋白、促进黏膜上皮细胞增殖有关。Objective： To observe the effect of Xiaobanxia Decoction （XBXT） on gastrointestinal mucosal barrier of rats after chemotherapy and to investigate the mechanism of XBXT on Gastrointestinal mucositis （GIM）. Methods： Male Wistar rats were randomly divided into normal control group, XBXT control group, cisplatin model group, omeprazole group, and low, medium and high doses of XBXT group. Rats in the first two control groups were given i.p.0. 9% NaCl （ 10 ml/kg） , while rats in other groups received ip 6 mg/kg cisplafin to have Gastrointestinal mucositis. Rats were given distilled water （10 ml/kg） ,0.8 g/kg XBXT, distilled water （10 m//kg）, omeprazole 1.2 mg/kg,0.4 g/ kg XBXT,0.8 g/kg XBXT, 1.6 g/kg XBXT in two days before i.p. cisplatin respectively. 24 and 72 h after established model 8 rats were selected in each group and the blood, gastric antrum and ileum were collected. Hematoxylin and eosin （HE） staining was used to observe the changes of gastric antrum and ileum. ELISA kit was used to measure the levels of MUC2, MUC5ac and D-Lactate in serum,and immunohistochemistry was used to measure the levels of MUC2 in ileum, MUC5ac in antrum and the Ki-67 in gastrointestinal mucosal cells. Results： In model rats, the levels of MUC2 and MUC5ac decreased, D-lactate increased in serum, proliferation rate of gastrointestinal mucosa cells and the mucin expression decreased （ P 〈 0.05 ）. XBXT increased the mucin in serum and tissue, and the cell proliferation rate （ P 〈 0.05 ）. Conclusion： 3.2 g/kg XBXT can inhibit the gastrointestinal mucositis ,thus the mechanism of XBXT may be related to the protection in gastrointestinal mucosal barrier.

关 键 词：小半夏汤 胃肠黏膜损伤 胃肠黏膜屏障 黏蛋白 

分 类 号：R285.5[医药卫生—中药学]