原花青素对特非那定诱导的斑马鱼心脏损伤的保护作用  

Protective effect of procyanidins to terfenadine-induced cardiac injury on zebrafish embryos

作　　者：李智平[1,2] 韩利文[2] 何秋霞[2] 党立[2] 韩建[2] 王希敏[2] 田青平[1] 刘可春[2] 

机构地区：[1]山西医科大学药学院,太原030001 [2]山东省科学院生物研究所山东省科学院药物筛选技术重点实验室,济南250014

出　　处：《中药药理与临床》2017年第3期54-57,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：山东省自主创新及成果转化专项(2014ZZCX02105);山东省重点研发计划项(2016GSF121009)

摘　　要：目的:研究原青花素对特非那定诱导斑马鱼的心脏损伤的保护作用,为原花青素的心脏保护作用的开发提供依据。方法:选取发育正常的48hpf(hour post fertilization)AB系斑马鱼胚胎,以0.5、1、3和5mg/L的原花青素对用特非那定(0.94mg/L)诱导斑马鱼心脏形态和功能影响的考察,在72hpf时,观察斑马鱼的心脏相关的指标,包括心脏形状、心囊大小、血液循环、血细胞在心区堆积等情况,测量斑马鱼的心率、测量静脉窦-动脉球(SV-BA)间距,以及主动脉的血流速度。结果:特非那定处理斑马鱼后,斑马鱼心脏出现了心脏形态、心率、功能等改变。原花青素达到1mg/L时可明显改善心脏泵血和全身血流速度等功能方面的作用;原花青素增加到3 mg/L时可明显缩短斑马鱼的SV-BA间距,缓解血细胞在心区堆积、心囊水肿等形态学的变化;原花青素增加至5mg/L时可明显提升斑马鱼心率。结论:原花青素对斑马鱼心脏功能损伤具有一定的保护作用,本实验为原花青素在心保护性作用药物的开发提供依据。Objective： To explore protective effect of procyanidins to terfenadine-induced cardiac injury on zebrafish embryos. Methods： Normal healthy48 hpf（ hour past fertilization） AB line zebrafish embryos were treated with 0.94mg/L terfenadine and produced cardiac injury model. Cardiac protective effects of procyanidins （0.5, 1,3, 5 rag/L） were researched by simultaneously treating 24 h zebrafish embryos with terfenadine. Each zebrafish was observed and recorded heart rate, the distance of venous sinus-artery （SV-BA） and the blood flow velocity of zebrafish aorta at 72 hpf. Results： Terfenadine-induced zebrafish heart showed serious cardiac injury; Compared with the terfenadine-induced group, treatment with procyanidins （ 1 mg/L） improved significantly the blood flow velocity of zebrafish aorta. Treatment with procyanidins （3 mg/L） could effectively relieve the interval of SV-BA and in blood cell accumulation, heart capsule edema. Besides, treatment with procyanidins （5 mg/L） could significantly increase heart rate. Conclusions： Procyanidins might be able to improve terfenadine-induced cardiac injury of zebrafish embryos, which was helpful to provide the reference for the development of cardioprotective drug.

关 键 词：原花青素 特非那定 斑马鱼 血液循环 心脏保护 

分 类 号：R285.5[医药卫生—中药学]