陈皮及其主要活性成分对脾虚模型大鼠血清胃泌素、血浆乙酰胆碱、P物质、胃动素和血管活性肠肽的影响  

Effect of Pericarpium Citri Reticulatae and its main active ingredients on gastrin in serum,acetylcholine,motilin,substance P and vasoactive intestinal peptide in plasma of rats

作　　者：宋玉鹏[1] 陈海芳[1] 胡源祥 谭舒舒 罗小泉[1] 杨武亮[1] 

机构地区：[1]江西中医药大学现代中药制剂教育部重点实验室,南昌330004

出　　处：《中药药理与临床》2017年第3期79-83,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目(81460575;81260612);江西中医药大学校级研究生创新项目(JZYC15S43)

摘　　要：目的:考察陈皮及其主要活性成分对脾虚模型大鼠血清胃泌素(GAS)、血浆乙酰胆碱(ACh)、P物质(SP)、胃动素(MTL)和血管活性肠肽(VIP)的影响。方法:以苦寒泻下加饥饱失常法复制大鼠脾虚模型,陈皮水煎液、橙皮苷、辛弗林及4个单体组合物(橙皮苷、辛弗林、川陈皮素、橘皮素)分别灌胃给药,采用ELISA法检测血清GAS、血浆ACh、SP、MTL和VIP的含量变化,考察陈皮水煎液及其主要活性成分对脾虚模型大鼠的影响。结果:模型组大鼠ACh、GAS、MTL和SP含量较空白对照组明显降低,而VIP含量则显著增加。给药后,吗丁啉6 mg/kg组GAS和MTL水平较模型组显著升高;橙皮苷12.68 mg/kg剂量组血清中GAS含量与模型组比较显著增加;橙皮苷12.68、25.36 mg/kg剂量组血浆中ACh含量,6.34 mg/kg剂量组血浆中SP含量,6.34、12.68、25.36 mg/kg剂量组血浆中MTL含量以及6.34 mg/kg剂量组血浆中VIP含量较模型组明显降低。辛弗林8.4 mg/kg剂量组血浆中ACh和MTL水平较模型组显著增加;辛弗林2.1 mg/kg剂量组血浆中SP含量和8.4 mg/kg剂量组血浆中VIP含量与模型组比较明显降低。4个单体组合物46.36 mg/kg剂量组血清中GAS、血浆中ACh以及SP含量较模型组比较显著增加;4个单体组合物11.59mg/kg剂量组血清中GAS、血浆中ACh、SP含量以及四个单体组合物11.59、23.18、46.36 mg/kg剂量组血浆中MTL含量与模型组比较明显降低。陈皮水煎液1.05 g/kg剂量组血清中GAS含量,4.2 g/kg剂量组血浆中ACh和SP含量与模型组比较明显增加;陈皮水煎液1.05、2.1 g/kg剂量组血浆中MTL和VIP含量较模型组明显降低。结论:橙皮苷和辛弗林是陈皮的药效物质。橙皮苷可能通过升高GAS的含量和抑制ACh、SP、MTL和VIP的分泌来促胃肠运动;辛弗林可能通过增加ACh和MTL的分泌和降低SP和VIP的含量来促胃肠运动;陈皮可能通过升高ACh、GAS和SP的含量和抑制MTL和VIP的分泌来促胃肠运动,这可能�Objective： To study the effect of Pericarpium Citri Retieulatae and its main active ingredients on gastrin in serum, acetylcholine, motilin, substance P and vasoactive intestinal peptide in plasma of rats. Methods ： The model was induced by filling out xie remedy to the case every other day. To investigate the effect of hesperidin, synephrine, monomer composition （ hesperidin, synephrine, nobiletin and tangeretin） and Periearpium Citri Reticulatae on model rats, the content change of aeetylcholine （ACh）, gastrin （GAS）, motilin （MTL）, substance P （SP） and vasoacfive intestinal peptide （VIP） were determined respectively. Results： The levels of ACh, GAS, MTL and SP in normal group increased, and VIP in normal group significantly decreased compared with model group （P 〈 0.01, P 〈 0.05）. After administration, the level of GAS in serum and the level of MTL in plasma in Dompefidone 6 mg/kg group increased significantly compared with model group （P 〈 0.05） ; The level of GAS in serum in 12.68 mg/kg group of hesperidin increased significantly compared with model group （P 〈 0.01 ） ; The levels of ACh in plasma in 12.68 and 25.36 mg/kg groups, SP in plasma in 6.34 mg/kg group, MTL in plasma in 6.34, 12.68 and 25.36 mg/kg groups, and VIP in plasma in 6.34 mg/kg group of hesperidin decreased significantly compared with model group （ P 〈 0.05, P 〈 0.01 ）. The levels of ACh and MTL in plasma in 8.4 mg/kg group of synephrine significantly increased compared with model group （ P 〈0.05, P 〈0.01 ） ; The levels of SP in plasma in 2.10 mg/kg group and VIP in plasma in 8.4 mg/kg group of synephrine significantly decreased compared with model group （ P 〈 0.01, P 〈 0.05）. The levels of GAS in serum, ACh and SP in plasma in 46.36 mg/kg group of monomer composition increased significantly compared with model group （ P 〈 0.05, P 〈 0.01 ） ; The levels of GAS in serum, ACh and SP in plasma in 11.59 mg/kg group, and MTL in plasma in 11.59, 23.18 and 46.36 mg/kg

关 键 词：陈皮 橙皮苷 川陈皮素 橘皮素 辛弗林 乙酰胆碱 胃泌素 胃动素 P物质 血管活性肠肽 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]