坤舒康颗粒对大鼠慢性盆腔炎的治疗作用及对子宫FGF-2、IGF-1 mRNA表达的影响  

Effects of Kunshukang Keli on chronic pelvic inflammatory disease in rats and expressions of FGF-2 and IGF-1 mRNA in uterus

作　　者：任艳青[1] 苏红宁[2] 冯梅梅[2] 张红竹 刘姣[1] 

机构地区：[1]河北中医学院药学院中药药理教研室,河北省中药配方颗粒工程技术研究中心,050200 [2]石家庄市第四医院,石家庄050011

出　　处：《中药药理与临床》2017年第3期152-155,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：河北省中医药管理局科研计划项目(2015206);河北省中医药管理局科研计划项目(2014007);河北省教育厅中药学教学实验示范中心建设项目(2015052265)

摘　　要：目的:研究坤舒康颗粒对大鼠慢性盆腔炎的治疗作用,探讨其可能的作用机制。方法:采用苯酚胶浆注射合并机械损伤的方法制备大鼠慢性盆腔炎模型,实验共分6组:假手术组,模型组,坤舒康颗粒5、10、20g/kg组,阳性药妇科千金片组。药物治疗20天后,计算各组大鼠子宫系数;检测血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、一氧化氮(NO)水平及血液流变学指标:全血及血浆粘度、红细胞压积;RT-PCR法检测子宫组织成纤维细胞生长因子-2(FGF-2)、胰岛素样生长因子-1(IGF-1)mRNA表达。结果:与模型组相比,5g/kg坤舒康颗粒可明显降低血清炎性因子TNF-α水平,红细胞压积,全血高、中切粘度;10g/kg坤舒康颗粒显著降低盆腔炎大鼠的子宫肿胀程度,TNF-α、IL-1β水平及各项血液流变学指标的异常;20g/kg坤舒康颗粒除了上述指标的明显改善外,还可显著降低血清NO含量;各剂量组均可明显降低子宫FGF-2、IGF-1 mRNA表达。结论:坤舒康颗粒可显著改善慢性盆腔炎大鼠的子宫肿胀与粘连,显著改善炎症反应及血液流变学异常,其作用机制与下调子宫FGF-2、IGF-1的表达密切相关。Objective： To study effects and possible mechanism of Kunshukang Keli on chronic pelvic inflammatory disease （CPID） in rats. Methods： Rat model of CPID was made with phenol paste injection combined with mechanical damage. Rats were randomly divided into six groups： the control group, the model group, Kunshukang Keli groups （5,10,20 g/kg） and Qianjinpian group. After intragastric administration for 20 days, the uterus weight coefficient was calculated, levels of tumour necrosis factor-α （TNF-α） , interleukin-1β （IL-1β） and nitric oxide （NO） in serum were examined, hemorheology indexes： blood and plasma viscosity, hematocrit were measured, expressions of fibroblast growth factor-2 （FGF-2） and insulin-like growth factor-1 （IGF-1） mRNA in uterine tissue were detected by RT-PCR. Results： Compared with the model group, Kunshukang Keli （5g/kg） obviously reduced levels of TNF-α, hematocrit, high and medium whole blood viscosity （P 〈0.05 or P 〈 0.01 ）. Kunshukang Keli （10g/kg） significantly reduced the degree of uterine swelling in CPID rats, levels of TNF-α and IL-1β, also ameliorated the abnormal hemorheology indexes （P 〈 0.05 or P 〈 0.01 ）, in addition obviously improved above indexes. Kunshukang Keli （20g/kg） also obviously reduced the content of NO （ P 〈0.01 ）. All doses of Kunshukang Keli decreased the mRNA expressions of FGF-2 and IGF-1 in uterus （ P 〈 0. 01 ）. Conclusion： Kunshukang Keli can significantly improve the uterine swelling and adhesion in CPID rats, ameliorate the inflammatory response and abnormal hemorheology, and the mechanism might be related to down-regulating the expression of FGF-2 and IGF-1 in uterus.

关 键 词：坤舒康颗粒 慢性盆腔炎 成纤维细胞生长因子-2 胰岛素样生长因子-1 

分 类 号：R285.5[医药卫生—中药学]