拟南芥开花诱导基因FT的蛋白表达及纯化  

Protein Expression and Purification of Flowering Inducer Gene FT in Arabidopsis thaliana

作　　者：袁敏 邢朝斌 葛伟娜 王莉 郭棣 

机构地区：[1]华北理工大学,生命科学学院,基因组学与计算生物学研究中心,唐山063000

出　　处：《基因组学与应用生物学》2017年第8期3053-3056,共4页Genomics and Applied Biology

基　　金：国家自然科学基金(31401212);河北省自然科学基金(C2014209134)共同资助

摘　　要：FT蛋白是植物的成花素,在植物多条不同开花途径的交汇节点发挥重要作用。该蛋白在植物叶片中合成运输到顶端分生组织发挥作用。为了研究FT蛋白的运输机制,本研究利用原核表达技术体外诱导表达了His6-FT蛋白;利用His镍柱纯化获得了高质量的His6-FT融合蛋白;利用Western blotting免疫印迹技术确定该His6-FT蛋白能够被anti-His单克隆抗体特异识别。试验获得的正确融合的His6-FT蛋白为进一步研究FT蛋白的转运机制奠定了良好的基础。FT protein is the florigen for plants, which plays an very important role in the intersection of many flowering pathways in plants. It is synthesized in leaves and transported to the apical meristem to perform its function. In order to study the transport mechanism of FT protein, the prokaryotic expression technique was used in this study to induce the expression of His6-FT protein in vitro. The high quality His6-FT fusion protein was purified by His purification method. The purified His6-FT recombinant protein could be detected by anti-His monoclonal antibody by using Western blotting immunoblotting technique. The properly fused His6-FT protein obtained in this study would lay a good foundation for further study of the transport mechanism of FT protein.

关 键 词：成花素 FT 融合蛋白 

分 类 号：Q943.2[生物学—植物学]