银屑病患者嗜酸性粒细胞P物质及受体神经激肽/速激肽受体1水平升高  

Increased expressions of substance P and neurokinin/tachykinin receptor 1 in eosinophils of patients with psoriasis

作　　者：左哲 王君灵 张慧云[1,2] 郑文娇[3] 张泽南 何韶衡 

机构地区：[1]锦州医科大学附属第一医院变态反应与临床免疫研究中心,辽宁锦州121001 [2]苏州市相城人民医院中心实验室,江苏苏州215100 [3]淄博矿业集团有限责任公司中心医院检验科,山东淄博255120

出　　处：《细胞与分子免疫学杂志》2017年第7期966-970,共5页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81172836;81471592;81472016)

摘　　要：目的研究银屑病患者外周血嗜酸性粒细胞P物质(SP)及其受体神经激肽/速激肽受体1(NK1R)的表达。方法流式细胞术检测银屑病患者和健康人外周血SP和NK1R的水平;用(0.1、1)μg/m L的蒿草花粉、梧桐花粉和尘螨过敏源粗提液刺激后,流式细胞术检测银屑病患者外周血嗜酸性粒细胞SP和NK1R的水平。结果银屑病患者SP+和NK1R+嗜酸性粒细胞的比例分别升高2.7倍和0.5倍,平均荧光强度分别增加1.5倍和0.2倍。此外,1μg/m L梧桐花粉过敏源粗提液刺激后,SP+嗜酸性粒细胞的百分比下调60%,而0.1μg/m L梧桐花粉过敏源粗提液刺激后NK1R+嗜酸性粒细胞的比例升高0.6倍。结论银屑病患者血液嗜酸性粒细胞SP和NK1R水平升高。Objective To investigate the expressions of substance P（ SP） and its receptor neurokinin/tachykinin receptor 1（ NK1R） in peripheral blood eosinophils of patients with psoriasis. Methods The levels of SP and NK1 R in the peripheral blood of both patients with psoriasis and healthy people were detected by flow cytometry. This method was again used to detect the levels of SP and NK1 R in the peripheral blood eosinophils of patients with psoriasis after stimulated with the crude extracts of Artemisia pollen,dust mite and Platanus pollen（ all at concentrations of 0. 1 and 1. 0 μg/m L）. Results Compared with the healthy controls,the percentages of SP^＋and NK1R^＋ eosinophils in psoriasis patients increased up to 2. 7 and0. 5 folds,respectively. Moreover,the mean fluorescence intensity（ MFI） of SP＋and NK1R^＋eosinophils of psoriasis patients were elevated by 1. 5 and 0. 2 folds,respectively. The percentage of SP^＋eosinophils in psoriasis were down-regulated by60% after the stimulation with Platanus pollen extract（ 1 μg/m L）,while 0. 1 μg/m L Platanus pollen extract induced a 0. 6-fold increase in the percentage of NK1R^＋eosinophis. Conclusion The expressions of SP and NK1 R are up-regulated in peripheral blood eosinophils of patients with psoriasis.

关 键 词：银屑病 嗜酸性粒细胞 P物质(SP) 神经激肽/速激肽受体1(NK1R) 

分 类 号：R446.61[医药卫生—诊断学] R758.63[医药卫生—临床医学]