黄芪甲苷干预高糖诱导的足细胞转分化的体外研究  

Efects of AS-IV on High Glucose Induced Podocyte Epithelial-mesenchymal Transition in Vitro

作　　者：陈廷芳[1] 郭永平[1] 桂定坤[1] 张海英[1] 汪年松[2] 

机构地区：[1]上海健康医学院附属第六人民医院东院,上海200200 [2]上海交通大学附属第六人民医院,上海200233

出　　处：《中国中西医结合肾病杂志》2017年第6期482-485,I0001,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：上海健康医学院种子基金资助项目(No.HMSF-16-22-013)

摘　　要：目的:观察黄芪甲苷对高糖诱导的足细胞转分化的影响。方法:将条件性永生的小鼠足细胞随机分为高糖组(HG)、正常对照组(NG)、甘露醇高渗对照组(MA)、及HG+不同剂量(5、15、30μg/ml)黄芪甲苷(AS-IV)干预组,采用免疫双标法、real time RT-PCR检测nephrin和desmin表达的变化。结果:高糖诱导足细胞转分化,desmin的表达增加,nephrin的表达减少。不同剂量黄芪甲苷可不同程度抑制高糖引起的上述改变。结论:高糖能诱导足细胞发生上皮-间充质转分化(EMT),黄芪甲苷能抑制高糖诱导的EMT。Objective： To observe the change of high glucose induced podocyte epithelial-mesenchymal transition（ EMT）,and AS-IV inhibit podocyte EMT. Methods： Conditionally immortalized mouse podocytes were randomly divided into 6 group： normal glucose group,high glucose group,mannitol group,HG ＋ 30 μg/ml AS-IV group,HG ＋ 15 μg/ml AS-IV group and HG ＋5 μg/ml AS-IV group. The expressions of desmin,nephrin were detected by a two-color immunohistochemistry staining technique and real time RT-PCR. Results： The expressions of desmin were enhanced and nephrin was decreased by high glucose,AS-IV markedly inhibited all of those changes induced by high glucose. Conclusion： The high glucose can induced podocyte EMT,and AS-IV might inhibit podocyte EMT.

关 键 词：足细胞转分化 糖尿病肾病 黄芪甲苷 

分 类 号：R285[医药卫生—中药学]