黄芩苷对哮喘大鼠气道重塑作用的实验研究  

Effect of baicalin on airway remodeling of bronchial asthma

作　　者：韩超[1] 杨柳[2] 张秋玲[1] 潘竞锵[1] 徐俊[2] 

机构地区：[1]广州市中医医院 [2]暨南大学药学院

出　　处：《中国临床药理学与治疗学》2017年第7期749-754,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：广东省中医药局基金项目(20141226);广州市中医药;中西医结合项目(20142A011001)

摘　　要：目的:初步探讨黄芩苷对支气管哮喘气道重塑的防治作用及其作用机制。方法:用卵清白蛋白(OVA)致敏大鼠制备支气管哮喘气道重塑动物模型,经黄芩苷干预治疗,用免疫组化法检测各组大鼠肺组织匀浆中α-平滑肌肌动蛋白(α-SMA)、磷酸化细胞外信号调节激酶1/2(pERK1/2)及气道组织中转化生长因子-β1(TGF-β1)蛋白的表达量;用RT-PCR检测气道平滑肌(ASM)中p-ERK1/2的mRNA水平。结果:实验结果显示,p-ERK1/2、α-SMA、TGF-β1蛋白的表达量在模型组中有明显的增加,地塞米松组、黄芩苷高剂量组和低剂量组的p-ERK1/2、α-SMA、TGF-β1蛋白的表达量均低于模型组(P<0.05,P<0.01)。结论:黄芩苷可降低TGF-β1、α-SMA的蛋白表达,并影响ERK信号传导通路,下调pERK1/2 mRNA含量,从而减轻气道炎症,延缓气道重塑。ABSTRACT AIM： To explore the effect and mechanism of baicalin on the airway remodeling of bronchial asthma. METHODS： Animal models of airway remodeling in asthmatic rats were induced by egg albumen （OVA）. After the treatment of Baicalin, expressions of TGF-β1, p-ERK1/2 and α-SMA in the lung tissue of rats were detected by immunohistochemical method, and the level of p-ERK1/2 mRNA in the airway smooth muscle （ASM） was detected by RT-PCR. RESULTS： Expressions of p- ERK1/2, α-SMA and TGF-β1 were significantly increased in the model group, which were all higherthan those of dexamethasone group, baicalin high dose group and low dose group （P 〈 0.05 or P 〈 0.01 ）. CONCLUSION： Baicalin can decrease the expression of TGF-β1, α-SMA, affect the ERK signal pathways, down regulate the content of p-ERK1/ 2 mRNA , thereby reducing airway inflammation and delaying airway remodeling.

关 键 词：黄芩苷 气道重塑 ERK信号通路 TGF-Β1 Α-SMA 

分 类 号：R965.2[医药卫生—药理学]