不同产地炒王不留行HPLC指纹图谱及含量测定研究  

Content determination and fingerprint of fried Vaccaria segetalis from different habitats by HPLC

作　　者：黄懿[1] 欧阳波[1] 肖作奇 潘涛[1] 

机构地区：[1]湖南省妇幼保健院,长沙410008

出　　处：《中南药学》2017年第7期879-882,共4页Central South Pharmacy

基　　金：湖南省中医药管理局科研项目(No.201627);长沙市科技局科技项目(No.K15ZD008-33)

摘　　要：目的采用高效液相色谱法建立炒王不留行的指纹图谱,同时对不同产地饮片中的王不留行黄酮苷含量进行测定。方法梯度洗脱,流速1.0 mL·min^(-1);流动相A为乙腈,流动相B为水,梯度洗脱程序为0~10.0 min,5%→10%A;10.0~85.0 min,10%→30%A;85.0~90.0 min,30%A;检测波长280 nm。结果建立了炒王不留行的HPLC指纹图谱,共发现14个共有峰,其中5号峰为王不留行黄酮苷,不同产地的炒王不留行中所含王不留行黄酮苷的含量在0.20%~0.45%。结论建立的炒王不留行HPLC指纹图谱可用于饮片质量控制,不同产地的饮片中王不留行黄酮苷含量不同。Objective To establish the HPLC fingerprint of fried Vaccaria segetalis, and determine the content of vaccarin. Methods The gradient elution solvent system consisted of acetonitrile-water solution at the flow rate of 1.0 mL · min^-1. The elution procedure was as follows： 0 - 10.0 min, 5%→10%A; 10.0 - 85.0 min, 10% → 30%A; and 85.0 - 90.0 min, 30%A. The detection wavelength was 280 nm. Results HPLC fingerprint of fried Vaccaria segetalis granules was established. Fourteen common peaks were selected as the fingerprint peaks, and peak 5 was vaccarin. The content of vaccarin in fried Vaccaria segetalis from different habitats was between 0.20% - 0.45%. Conclusion The HPLC fingerprint can be applied to control the quality of fried Vaccaria segetalis. The content of effective components from different habitats is different.

关 键 词：炒王不留行 高效液相色谱法 指纹图谱 含量测定 王不留行黄酮苷 

分 类 号：R284.1[医药卫生—中药学]