二甲双胍对异氟醚麻醉小鼠血糖的影响  

Effects of mefformin pretreatment on blood glucose level in mice under isoflurane anesthesia

作　　者：李秀秀[1] 赵鑫[1] 许芳霞[1] 邵学明 张朋[1] 马正良[1] 顾小萍[1] 

机构地区：[1]南京大学医学院附属鼓楼医院麻醉科,210008

出　　处：《国际麻醉学与复苏杂志》2017年第8期677-681,共5页International Journal of Anesthesiology and Resuscitation

基　　金：国家自然科学基金（81371207）

摘　　要：目的观察二甲双胍对异氟醚麻醉小鼠血糖的影响。方法156只8周龄雄性C57BL／6小鼠分两部分进行实验。①血糖检测：这部分实验分两步进行。首先，28只小鼠按照按随机数字表法分为未禁食组（n组）和禁食12h组（f组）；各组再分为2个亚组，对照组（Cn组／Cf组），麻醉组（An组／Af组）。进一步实验使用56只小鼠按随机数字表法分为n组和f组，每组28只。两组均再按随机数字表法分为4个亚组，Cn组／Cf组，An组／Af组，二甲双胍组（Mn组／Mf组）和二甲双胍麻醉组（MAn组脚Af组），每亚组均为7只。Mn组、Mf组、MAn组和MAf组于麻醉前1．5h腹腔注射二甲双胍40ms／kg，An组、Af组、MAn组和MM组均行异氟醚6h麻醉。通过尾静脉取血检测围麻醉期各时间点血糖值。②荧光定量核酸扩增检测系统（quantitativepolymerase chain reaction detecting system, Q-PCR）检测：共72只小鼠。各亚组分组方法同上，分别于麻醉1、4、6h取材下丘脑，每组每个时间点取3只。QTCR检测腺苷酸活化蛋白激酶（adenosine monophosphate activated protein kinase, AMPK）两个亚型AMPKα1、AMPKα2的mRNA表达水平。结果血糖水平变化：与n组比较，f组小鼠血糖显著降低[（8．25±1．02）mmol／L比（6．46±0．65）mmol／L，P〈0．05]。在f组和n组中，麻醉组小鼠均出现血糖水平升高；与cn组和cf组比较，An组和Af组分别在麻醉0．5、1、2h和1、2、3h升高（P〈0．05）；分别与Cn组和Cf组比较，Mn组和Mf组血糖水平差异无统计学意义（P〉0．05）；MAn组和MM组均于麻醉2h开始出现低血糖并持续（P〈0．05），这种差异分别在麻醉结束4h和1h后消失。下丘脑AMPKmRNA表达：1h，与cf组比较，MAf组AMPKα1和AMPKα2mRNA表达下调（P〈0．05）；4h，与cn组和cf组比较，An组、MAn组和Af组AMPKα1表达下调（P〈0．05），AMPKα2mRNA表达在MAf组降低（P〈0．05）；6h，与Cn组和Objective To examine the effects of metformin on blood glucose levels in mice under isoflurane anesthesia. Methods One hundred and fifty-six 8-week-old male C57BL/6 mice were used for blood glucose tests and AMPK mRNA measurement. ① Blood glucose test, there were two steps in this part： firstly, twenty-eight mice were randomly divided into two groups, subject to normal diet （n） or 12 h fasting （f） （n=14）; each group was further divided into two subgroups： no additional treatment （group Cn/group Cf）, anesthesia group （group An/group Af）. Secondly, fifty-six mice were randomly divided into two groups, subject to group n or group f （n=28）; each group was further divided into 4 subgroups： group Cn/group Cf, group An/group Af, metformin group （group Mn/group Mr）, metformin combined with anesthesia group （group MAn/group MAf） （n =7）. Metformin （40 mg/kg） was administered intraperitoneally 1.5 h before anesthesia （1.5%isoflurane, for 6 h）. The blood was collected from tail vein immediately before anesthesia and 0.5, 1, 2, 3, 4, 6 h after induction of anesthesia, and was used for glucose determination.② mRNA measurement, seventy-two mice were used for mRNA measurement. The mRNA levels of AMPKα1 and AMPKα2 in the hypothalamus were measured with quantitative polymerase chain reaction detecting system （Q-PCR） 1, 4 h and 6 h after the onset of anesthesia （3 mice at each time point for each subgroup）. Results Fasting reduced blood glucose levels, while metformin did not alter blood glucose levels in both n and f mice. Anesthesia increased blood glucose levels within the first 2-3 h. However, metformin maintained hypoglycemia in anesthetized mice. The levels of AMPK mRNA in the hypothalamus were altered by metformin and isoflurane depending on the duration of anesthesia. Metformin＋1 h anesthesia significantly attenuated mRNA levels of AMPKα1 and AMPKα2 （P〈0.05）. Four hours anesthesia alone and combined with metformin decreased AMPKα1 mRNA levels （P〈

关 键 词：异氟醚 血糖 二甲双胍 腺苷酸活化蛋白激酶 

分 类 号：R614[医药卫生—麻醉学]