“健脾和胃祛湿方”对大鼠肝组织CYP450酶作用和初步机制  

Effect of Jianpi Hewei Qushi Fang on activity and expression of CYP450 enzymes in liver tissue of rats

作　　者：吕雪幼 郭利华[1] 

机构地区：[1]宁波大学医学院附属医院消化内科,浙江省宁波市315020

出　　处：《世界华人消化杂志》2017年第22期2022-2027,共6页World Chinese Journal of Digestology

摘　　要：目的从分子水平探讨"健脾和胃祛湿方"对药物代谢酶CYP450的作用.方法采用液质联用分析、Western blot及RTPCR方法测定大鼠肝微粒体及肝脏组织中CYP450酶系6种亚型(CYP1A2、CYP2C9、CYP2C19、CYP2D6、CYP2E1、CYP3A4)的活性、蛋白及mRNA表达水平的影响.结果"健脾和胃祛湿方"对CYP450亚型CYP2C19、CYP2C9、CYP2D6、CYP2E1和CYP3A4的酶活性,蛋白及mRNA表达水平没有显著性影响;而呈剂量依赖性地降低CYP1A2酶活性,蛋白及mRNA表达水平.结论"健脾和胃祛湿方"作用于药物代谢酶CYP450亚型CYP1A2酶活性、蛋白及mRNA表达水平,对CYP450酶其他亚型没有影响.AIM To investigate the effect of Jianpi Hewei Qushi Fang（JPHWQSF）, a traditional Chinese medicine recipe, on the activity and expression of CYP450 enzymes in liver tissue of rats.METHODS The activity and protein and mRNA expression of CYP450 enzyme subtypes（CYP1A2, CYP2C9,CYP2C19, CYP2C6, CYP2E1, CYP3A4） in liver tissue of rats were determined by liquid chromatography-mass spectrometry, Western blot and RT-PCR, respectively.RESULTS JPHWQSF treatment had on significant effect on the activity and protein and mRNA expression of CYP2C9, CYP2C19, CYP2C6, CYP2E1, and CYP3A4 in liver tissue of rats, but significantly decreased the activity and protein and mRNA expression of CYP1A2 in a dose-dependent manner.CONCLUSION JPHWQSF treatment alters the activity and protein and mRNA expression of CYP1A2, but has no effect on other subtypes of CYP450.

关 键 词：健脾和胃祛湿方 CYP450 CYP1A2 

分 类 号：R285.5[医药卫生—中药学]