基于ISSR技术和非腺毛特征研究柔毛淫羊藿和近缘种遗传关系和居群遗传多样性  

Genetic relationship and population genetic diversity of Epimedium pubescens and its related species based on ISSR and non-glandular hair characteristic

作　　者：刘翔[1,2] 付长珍[1] 高敏[1] 植汉成 杨相波 郭宝林[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]重庆市中药研究院,重庆400065 [3]江西中医药大学,江西南昌330006 [4]贵州同济堂制药股份有限公司,贵州贵阳550002

出　　处：《中国中药杂志》2017年第16期3090-3097,共8页China Journal of Chinese Materia Medica

基　　金：重庆市科委前沿与应用基础研究项目(cstc2014jcyjA10128);重庆市科委基本科研业务专项(2015cstc-jbky-01902)

摘　　要：该研究对柔毛淫羊藿14个居群和星花淫羊藿1个居群共128个单株进行ISSR分析,用POPGENE计算相关系数,UPGMA进行聚类分析,以及用光学显微镜观察了每个居群叶背主要非腺毛类型和特点。研究发现:非腺毛可分为5个形态类别,分别为长直毛、长卷曲毛、伏卷曲毛、拟短伏毛和长伏毛;筛选出的8个ISSR引物共扩增出94个条带,其中多态性条带90个,基于ISSR聚类分析结果,15个居群分为3个分支,星花淫羊藿居群并没有单独为一支,应不能独立成种,应并入柔毛淫羊藿或者作为柔毛淫羊藿的种下等级;柔毛淫羊藿居群间遗传关系与地理分布及非腺毛特征具有良好的相关性,但也存在不一致的情况,这对于进一步研究柔毛淫羊藿以及淫羊藿属植物的种间关系和成种方式提供了良好的提示。居群多样性分析表明柔毛淫羊藿居群的基因流N_m为0.354 4,Nei's基因分化系数为0.585 2,说明居群间表现出较高水平的遗传分化,主要原因可能来自柔毛淫羊藿居群自交率高和种子散布范围小。In this study,128 individuals form 14 Epimedium pubescens populations and 1 E. stellulatum population were analyzed by ISSR marker. The data were calculated by POPGENE software and clustered by UPGMA method. Optical microscope was used to observe the main types of the non-glandular hairs and their characteristics in each population. It is found that the following conclusions：Non-glandular hairs can be divided into five morphological categories,long straight pubescent,curly pubescent,appressed curly pubescent,pseudo short appressed hairs and long appressed. Eight primers were screening and a total of 94 bands were detected in ISSR,among which 90 were polymorphic bands. Based on the results of ISSR cluster analysis,15 populations were divided into 3 clades. E.stellulatum populations should be incorporated into the E. pubescens or as avariety under E. pubescens not be independent and as it has no separate phylogenetic branch for a cluster. The genetic relationship among the populations of E. pubescens was closely related with its geographical distribution and non-glandular hair features. But there were also some inconsistency,which provided a good hint for the further study on the interspecific relationship and natural speciation manner of Epimedium species. Population diversity analysis showed N_m= 0. 354 4,Nei＇s = 0. 585 2. It was showed that E. pubescens has high genetic diversity among populations,for which the main reason was probably the high inbreeding rate and the small range of seed dispersal.

关 键 词：柔毛淫羊藿 星花淫羊藿 居群 非腺毛 ISSR分子标记 遗传多样性 

分 类 号：S567.239[农业科学—中草药栽培]