水蛭通过p38MAPK信号通路对早期动脉粥样硬化大鼠VSMCs的影响  

Effect of leech on VSMCs in early atherosclerosis rats via p38MAPK signaling pathway

作　　者：吴晶魁 杨乔[1] 李洋洋[2] 谢东杰[1] 

机构地区：[1]天津医科大学总医院,天津300052 [2]河南科技大学第一附属医院,河南洛阳471003

出　　处：《中国中药杂志》2017年第16期3191-3197,共7页China Journal of Chinese Materia Medica

摘　　要：为了探究中药水蛭干预作用下p38MAPK信号转导通路对早期动脉粥样硬化(AS)大鼠血管平滑肌细胞(VSMCs)增殖与凋亡的影响及其可能机制。实验通过生化分析仪检测中药水蛭对大鼠血脂(TG,TC,LDL-C,HDL-C)水平的调控;ELISA检测血清中TGF-β1的水平;免疫组织化学法(IHC)检测VSMCs的PCNA,Caspase-3表达水平;Western blot检测中药水蛭对VSMCs中MKK3,p38及C-myc蛋白表达的影响;HE染色观察大鼠胸主动脉形态变化。结果显示,中药水蛭可使TC,LDL-C明显降低,HDL-C升高,TGF-β1,PCNA的表达水平明显下降,Caspase-3表达上调,MKK3,p38及C-myc蛋白表达水平下降,以及抑制内膜增厚、减少斑块形成。以上结果表明中药水蛭可能通过调降血脂,降低TGF-β1水平,影响p38MAPK信号通路蛋白表达,从而抑制VSMCs的增殖、促进其凋亡,抑制内膜增厚、减少斑块形成等环节,进而干预早期AS进程。To explore the effect of leech on proliferation and apoptosis of vascular smooth muscle cells（ VSMCs） in early atherosclerosis rats via p38 MAPK signaling pathway and investigate its possible mechanism. Biochemical analyzer was used to examine the regulation of leech on levels of triglycerides（ TG）,total cholesterol（ TC）,low-density lipoprotein（ LDL-C）,and high-density lipoprotein（ HDL-C） in blood lipid of rats. The expression of transforming growth factor-beta 1（ TGF-β1） in serum was detected by ELISA. Immunological histological chemistry（ IHC） was taken to measure the expression levels of proliferating cell nucleus antigen（ PCNA） and cell apoptosis proteinase-3（ Caspase-3）,while the protein expression levels of MKK3,p38 and C-myc were detected by Western blot.In addition,hematoxylin and eosin（ HE） staining was used to observe the morphological change of thoracic aortas. The results showed that leech decreased the levels of TC,LDL-C obviously and increased HDL-C,suppressed the expression levels of TGF-β1 and PCNA,up-regulated Caspase-3,down-regulated the expression levels of MKK3,p38,and C-myc protein. HE staining indicated that it could inhibit intimal thickening and reduce plaque formation. The above results indicated that leech may affect the protein expression of the p38 MAPK signaling pathway to inhibit proliferation and promote the apoptosis of VSMCs via reducing blood lipid levels and suppressing TGF-β1,aiming at inhibiting intimal thickening and reducing plaque formation,tand then slowing down the process of early atherosclerosis.

关 键 词：水蛭 动脉粥样硬化 P38MAPK信号通路 血管平滑肌细胞 增殖与凋亡 

分 类 号：R285.5[医药卫生—中药学]