腺苷预处理对腹部开放伤海水浸泡大鼠肠道黏膜的保护作用  

Protective effects of adenosine preconditioning on intestinal mucosa in rats with open abdominal injury coupled with seawater immersion

作　　者：浦江[1] 王少鑫[1] 王晓辉[1] 于兰[1] 罗哲[1] 黄泽平[1] 崔立红[1] 

机构地区：[1]海军总医院消化内科,北京100048

出　　处：《中华航海医学与高气压医学杂志》2017年第3期173-176,195,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

摘　　要：目的 观察腺苷预处理对腹部开放伤海水浸泡大鼠肠道炎症反应、黏膜组织结构破坏和肠道黏膜上皮细胞凋亡的影响.方法 选取60只雄性Wistar大鼠,按数字表法随机分为3组：A组,腹部开放伤0.9％氯化钠溶液浸泡＋尾静脉注射灭菌注射用水（3 mg/kg）;B组,腹部开放伤海水浸泡＋尾静脉注射灭菌注射用水（3 mg/kg）;C组,腹部开放伤海水浸泡＋尾静脉注射腺苷预处理（3 mg/kg）.每组20只.大鼠于浸泡6h后处死.观察肠道组织病理改变;应用酶联免疫法测定肠道黏膜肿瘤坏死因子（TNF）-α,白细胞介素（IL）-6含量;免疫组化检测Caspase-3蛋白;TUNEL法检测肠道黏膜凋亡细胞.结果 B组大鼠肠道黏膜病理损伤评分（3.98 ±0.41）较A组（2.46±0.39）显著升高（P＜0.01）;B组肠黏膜TNF-α和IL-6水平[（2.38±0.39） μg/L与（883.11 ±34.22） ng/L]较A组[（1.01 ±0.06） μg/L与（258.09 ±6.52） ng/L]显著升高（P＜0.01）,C组[（1.88±0.21） μg/L与（582.13±19.44） ng/L]较B组明显下降（P＜0.05）.B组小肠黏膜上皮中Caspase-3蛋白阳性率与凋亡细胞百分率[（27.9±4.3）％与（24.5±3.1）％]明显高于A组[（13.8±2.6）％与（16.4±2.3）％]（P＜0.01）,C组[（20.2±3.4）％与（19.9±2.9）％]较B组明显下降（P＜0.05）.结论 腹部开放伤海水浸泡大鼠应用腺苷预处理后肠道黏膜炎性反应明显缓解,减轻肠道黏膜组织结构的破坏,减少肠道黏膜上皮细胞的凋亡.Objective To observe the effects of adenosine preconditioning on the intestinal inflammatory response,the damaged mucosal tissue and apoptosis of intestinal epithelial cells in rats with abdominal open injury coupled with seawater immersion.Methods Sixty male Wistar rats with abdominal open injury were randomly divided into 3 groups,each consisting of 20 animals.The animals in group A had 0.9% sodium chloride solution immersion ＋ caudal vein injection of sterilization water （3 mg/kg）.The animals in group B received seawater immersion ＋ caudal vein injection of sterile water （3 mg/kg） and the animals in group C were given seawater immersion ＋ caudal vein injection of adenosine pretreatment （3 mg/kg）.All animals were sacrificed 6 hours after immersion.Pathological changes in intestinal mucosa were closely observed by histopathology.Levels of TNF-α and IL-6 were detected by enzyme-linked immunosorbent assay （ELISA）.Caspase-3 protein was detected by immunohistochemistry.Apoptosis of the cells in intestinal mucosa were monitored by TUNEL method.Results The intestinal pathological scores of TNF-α and IL-6 for the animals in group B（3.98 ±0.41）were significantly higher than those in group A （2.46 ±0.39） （P 〈0.01）.The TNF-α and IL-6 levels for the animals in group B [（2.38 ±0.39） μg/L and（883.11 ±34.22） ng/L] were significantly increased,as compared with those in group A [（1.01 ± 0.06） μg/L and （258.09 ± 6.52） ng/L] （P 〈 0.01）.The TNF-α and IL-6 levels for the animals in group C[（1.88 ± 0.21）pg/L and （582.13 ±19.44）ng/L] were significantly decreased,as compared with those in group B （P 〈 0.01）.The level of Caspase-3 and percentage of apoptotic cells in the intestinal mucosa of the animals in group B [（27.9 ± 4.3） % and （24.5 ± 3.1） %]were significantly higher than those of the animals in group A [（13.8 ± 2.6） % and （16.4 ± 2.3） %] （P 〈0.01）,and the expression level of Caspase-3 and percentage of apoptotic cells

关 键 词：腺苷预处理 腹部开放伤 海水浸泡 肠道黏膜 细胞凋亡 

分 类 号：R82[医药卫生—临床医学]