化香树果序乙醇提取物诱导CNE1、CNE2细胞发生methuosis死亡的机制  

Mechanism of Platycarya strobilacea Sieb. et Zucc extract-induced methuosis in human nasopharyngeal carcinoma CNE1 and CNE2 cells

作　　者：朱俊谕 涂维[1,3] 曾超[1,3] 毛珩旭 杜庆锋[1] 蔡红兵[1,3] 

机构地区：[1]南方医科大学,广东广州510515 [2]南方医科大学珠江医院,广东广州510282 [3]南方医科大学中西结合医院,广东广州510315

出　　处：《南方医科大学学报》2017年第6期827-832,共6页Journal of Southern Medical University

基　　金：广东省自然科学基金(2016A030313724);广东省中医药局科研项目(20173028)

摘　　要：目的探讨化香树果序乙醇提取物(PSZ)诱导鼻咽癌细胞CNE1、CNE2发生methuosis死亡的机制。方法用1.0 mg/m L PSZ处理CNE1、CNE2细胞,观察细胞形态学变化,RT-q PCR、Western blotting检测PSZ干预后Rac1基因和蛋白表达水平。EHT 1864抑制两组细胞Rac1表达,观察化香树果序醇提物对CNE1、CNE2细胞的作用。结果 CNE1、CNE2细胞经PSZ处理后,细胞质出现大量空泡,空泡相互融合,不断增大,最后细胞膜破裂,细胞核没有发生明显的变化,这种形态学改变符合methuosis细胞死亡方式,Rac1在基因和蛋白水平表达均增加。EHT 1864抑制细胞Rac1表达后,PSZ引起的methuosis死亡现象消失。结论 Rac1是PSZ诱导人鼻咽癌细胞CNE1、CNE2发生methuosis死亡的关键靶点。Objective To study the effect of Platycarya strobilacea Sieb. et Zucc （PSZ） extract on methuosis of human nasopharyngeal carcinoma CNE1 and CNE2 cells and explore the underlying mechanism. Methods CNE1 and CNE2 cells were treated with 1 mg/mL PSZ extract and the expressions of Rac1 mRNA and Rac1 protein were detected using RT-qPCR and Western blotting, respectively. Results CNE1 and CNE2 cells showed obvious morphological changes typical of methuosis following treatment with PSZ extract characterized by cell merging, accumulation of large cytoplasmic vacuoles, and membrane rupture without obvious changes in the nuclei. PSZ treatment resulted in up-regulated Rac1 mRNA and Rac1 protein expressions in the cells. Application of EHT 1864 obviously blocked the effect of PSZ extract in inducing methuosis in CNE1 and CNE2 cells. Conclusion PSZ extract can induce methuosis in CNE1 and CNE2 cells by inducing the overexpression of Rac1.

关 键 词：鼻咽癌 化香树果序乙醇提取物 methuosis RAC1 

分 类 号：R285[医药卫生—中药学]