头孢霉素与卡那霉素对花生组培苗生根的影响  

Effects of Cephalosporin and Kanamycin on Rooting of Peanut Tissue Culture Seedlings

作　　者：阮建[1] 郭峰[2] 李新国[2] 史灵敏 田海莹 张会[3] 万书波[1,2] 彭振英[1,2] 

机构地区：[1]山东大学生命科学学院,山东济南250100 [2]山东省农业科学院生物技术研究中心/山东省作物遗传改良与生态生理重点实验室,山东济南250100 [3]新疆农业大学农学院,新疆乌鲁木齐830000

出　　处：《山东农业科学》2017年第8期33-37,共5页Shandong Agricultural Sciences

基　　金：国家自然科学基金项目(31571605);国家科技支撑计划项目(2014BAD11B04);国家国际科技合作专项(2015DFA31190);现代农业产业技术体系建设专项(CARS-14)

摘　　要：抗性筛选是农杆菌介导的花生遗传转化过程中不可或缺的步骤,但是抗生素的使用对花生组培苗的生长与生根有着显著影响。为了明确抗生素对花生组培苗生根的敏感质量浓度,本试验以花生栽培品种丰花1号组培苗为材料,在培养基中添加不同浓度的头孢霉素与卡那霉素,检测花生组培苗的生根率及植株生长状况并进行统计分析。结果表明:0~250 mg/L的头孢霉素对花生组培苗生根及植株生长基本无影响。卡那霉素对花生组培苗生根影响较为显著,0~10 mg/L卡那霉素对花生组培苗生根与生长基本无影响,10 mg/L卡那霉素条件下组培苗不长侧根,15 mg/L卡那霉素显著抑制根的伸长;切去不生根的组培苗底端后移入MS培养基又能重新生根,不切去底端的组培苗则不生根。经过75 mg/L卡那霉素筛选的抗性组培苗的生根率可达31%。本研究可为花生遗传转化后期根的诱导提供一定的理论支持。Resistance screening is an indispensable step in Agrobacterium mediated genetic transformation of peanut,but the use of antibiotics have significant influence on peanut seedling growth and rooting. In order to make clear the sensitive concentration of antibiotics on rooting of peanut seedlings,the peanut cultivation variety Fenghua 1 tissue culture seedlings were used as experimental materials,and the culture media with different concentrations of kanamycin or cefotaxime were used to detect the peanut seedling rooting rate and growth status. The results showed that 0 ~ 250 mg/L cefotaxime had little effect on peanut growth,while kanamycin had significant effect on peanut seedling rooting. 0 ~ 10 mg/L kanamycin has no obvious effect on peanut seedling rooting and growth,but under the condition of 10 mg/L kanamycin,the seedlings had no branch roots,what＇s more,15 mg/L kanamycin significantly inhibited the elongation of roots. When the rootless plantlets were transfered into MS medium after cutting off the bottom of the seedlings,the roots grew out again,whereas the rootless plantlets did not grow roots if not cutting off the bottom. After screening with 75 mg/L kanamycin,the rooting rate of the kanamycin-resistant tissue culture plantlets could reach 31%. This study could provide certain theoretical supports for roots induction during the late stage of peanut genetic transformation.

关 键 词：头孢霉素 卡那霉素 花生 组培苗 生根率 遗传转化 

分 类 号：S565.201[农业科学—作物学]