白刺花染色体压片技术优化及核型分析  

Optimization of Chromosome Preparation and Karyotype Analysis of Sophora davidii

作　　者：雷文英[1] 赵丽丽[1] 简忠领 陈超[1] 宋高翔[1] 

机构地区：[1]贵州大学动物科学学院草业科学系,贵阳550025

出　　处：《种子》2017年第8期5-9,共5页Seed

基　　金：贵州大学大学生创新创业训练计划项目(创新020);国家科技支撑计划(2014BAD 23B03);国家自然科学基金项目(31560664);贵州省科技计划项目(黔科合支撑[2016]2516号;黔科合NY字[2014]3048号);黔南州社会发展科技项目(喀斯特石漠化林草牧综合治理技术研究与示范)

摘　　要：以贵州白刺花(Sophora davidii)根尖为试材,从取材时间、预处理方法、解离时间3个方面改进白刺花染色体常规压片技术,并对白刺花染色体进行核型分析。结果表明,白刺花染色体压片最佳取材时间为09:00-10:00时,根尖用冰水混合物在4℃冰箱里预处理24h为宜,卡诺固定液(无水酒精∶冰醋酸=3∶1)固定24h,解离用1mol/L盐酸在60℃水浴下解离8min最好。核型分析结果表明,白刺花染色体基数为x=9,染色体数目2n=18,染色体核型公式为2n=2x=2M+16m,染色体相对长度为2n=2x=18=2S+10M1+6M2。白刺花的染色体属于1A型。To optimize the chromosome preparation procedure and investigate the karyotype of Sophora davidii.The root of Sophora davidii growing in Guizhou was used as plant material.Factor affected chromosome preparation including sampling time,pretreatment and dissociation durations were investigated.Results showed that, The best compression technique for Sophora davidii chromosome was as follows： sampling time for 09：00-10：00 in the morning,24 hour pretreatment using mixture of ice and water in the refrigerator at 4℃, 8 minute dissociation at 60 ℃ using hydrochloric ac{d 1 mol/L.The basic chromosome number was x= 9, Sophora davidii was diploid and the chromosome number was 2 n= 18, the chromosome karyotype formula was 2 n= 2 x= 2 M＋16 m, the relative length of the chromosome was 2n=2 x= 18=2 S＋10 M1＋6M2.The chromosome of Sophora davidii belongs to 1 A type.

关 键 词：白刺花 染色体压片 染色体数目 核型分析 

分 类 号：S793.2[农业科学—林木遗传育种]