基于成熟期的梨品种遗传多样性SSR分析  

Genetic Diversity of SSR Analysis of Pear Cultivars Based on Maturity

作　　者：赵思思[1] 刘兴菊[1] 李雪雁[1] 王晓叶[1] 梁海永[1] 刘铭[1] 

机构地区：[1]河北农业大学林学院,河北省林木种质资源与森林保护重点实验室,河北保定071000

出　　处：《北方园艺》2017年第16期43-49,共7页Northern Horticulture

基　　金：国家林业公益性行业资助项目(201104039);国家林业局科技发展中心资助项目(XPC-201505)

摘　　要：以63个梨主栽品种为试材,采用SSR分子标记技术与毛细管电泳技术相结合的方法,研究了梨不同成熟期的种质资源遗传多样性,以期为梨栽培品种的品种鉴别、亲子关系鉴定、分类及系统关系研究、遗传图谱构建与标记辅助育种在分子水平上提供参考依据。结果表明:3份梨种质的观测等位基因数(Na)平均为14.8,有效等位基因数(Ne)平均为7.363 1,Shannon多样性指数(I)平均为2.195,期望杂合度(Nei)平均为0.845,说明梨总群体遗传变异偏高。依据梨系统将其分为白梨、秋子梨、日本梨、砂梨和西洋梨5个类群,其中白梨系统在Shannon多样性指数和期望杂合度上最高,分别为2.069 9和0.831 1,秋子梨系统最低,分别为0.420 8和0.303 6,白梨与砂梨类群的遗传一致度最高为0.715 2。Sixty-three main varieties of Pyrus L.were used as experimental materials.The method of combination with SSR molecular marker technique and capillary electrophoresis technique was used to study the genetic diversity of Pyrus L.varieties in order to supply reference for breed identification,parent-child relationship identification,classification and systematic research,genetic map construction and marker-assisted breeding of Pyrus L.The results showed that,the average number of alleles（Na）was 7.363 1,the Shannon diversity index（I）was 2.195,the expected heterozygosity（Nei）average of0.845,indicating that the total genetic variation of the pear population was high.According to the pear system,it was divided into five groups：white pear,autumn pear,Japanese pear,Chinese pear and European pear.The white pear system was the highest in Shannon diversity index and expected heterozygosity,which were 2.069 9and 0.831 1,respectively.The lowest were respectively 0.420 8and 0.303 6,white pear and sand pear group genetic consistency of the highest,was 0.715 2.

关 键 词：梨 简单重复序列(SSR) 毛细管电泳 成熟期 遗传多样性 

分 类 号：S661.203.6[农业科学—果树学]