青天葵总黄酮抗氧化及抑菌活性  

Antioxidant and Antibacterial Activity of Total Flavonoids FromNervilia fordii

作　　者：许海棠[1] 李浩[1] 赵彦芝[1] 周菊英[1] 杨立芳[1] 马少妹[1] 

机构地区：[1]广西民族大学化学化工学院,广西林产化学与工程重点实验室,广西南宁530008

出　　处：《北方园艺》2017年第16期161-165,共5页Northern Horticulture

基　　金：国家自然科学基金资助项目(81560713);国家民委资助项目(14GXZ012);广西高校科学资助项目(KY2015YB080)

摘　　要：以青天葵为试材,乙醇为溶剂,对青天葵进行总黄酮提取;采用清除羟自由基、DPPH自由基、超氧阴离子自由基和测定总还原能力的方法来评价青天葵总黄酮抗氧化活性;并通过测定其对6种试验菌的最低抑菌浓度来考察其抑菌活性。结果表明:青天葵黄酮提取液对羟自由基的清除优于同浓度的抗坏血酸溶液,清除DPPH自由基的IC50是17.4μg·mL^(-1),清除超氧阴离子自由基的IC50是95.5μg·mL^(-1),还原能力稍弱于抗坏血酸;对白色念珠球菌、铜绿假单胞菌和肺炎克雷伯氏菌的抑制作用较强,最低抑菌浓度均为0.25mg·mL^(-1)。Nervilia fordii was used as test material,total flavonoids were extracted from Nervilia fordii using ethanol as extraction solvent.The antioxidant activity was evaluated by·OH,DPPH·,O2~ radical scavenging assays and reducing power assay.And the antibacterial effects were investigated by its minimal inhibitory concentrations（MIC）for six test bacterias.The results showed that the extract from Nervilia fordii showed strong scavenging ability on hydroxyl radical,which was better than that of ascorbic acid solution at the same concentration.The IC50 of scavenging DPPH radical was 17.4μg·mL^-1,IC50 of scavenging O2~radical was 95.5μg·mL^-1,and reducing power was relatively weak.The results of antibacterial tests indicated that total flavonoids from Nervilia fordii had good antibacterial ability on Monilia albican,Pseudomonas aeruginosa and Klebsiella pneumoniae,with MIC of 0.25 mg·mL^-1.

关 键 词：青天葵 总黄酮 抗氧化活性 抑菌活性 

分 类 号：S567.239[农业科学—中草药栽培]