HIF-1α介导BMSCs复合PLGA修复大鼠颅骨标准骨缺损的研究  

Effects of HIF-1α transfected BMSCs combined with PLGA on repairing rat calvarial bone defects

作　　者：朱艳秋[1,2] 王默涵 周咏[1,2] 许敏[1,2] 何家才[1,2] 

机构地区：[1]安徽医科大学口腔医学院,合肥230032 [2]安徽医科大学附属口腔医院安徽省口腔疾病研究中心实验室,合肥230032

出　　处：《安徽医科大学学报》2017年第9期1261-1265,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81371114);安徽省自然科学基金(编号:1408085MKL29);安徽省科技攻关项目(编号:1604a0802082)

摘　　要：目的构建低氧诱导因子(HIF)-1α介导骨髓间充质干细胞(BMSCs)复合聚乳酸-聚乙醇酸共聚物(PLGA)支架材料血管化组织工程骨,观察其在大鼠颅骨标准骨缺损中的修复效果。方法将目的基因HIF-1α转染至BMSCs后,与支架材料PLGA复合,修复SD大鼠颅骨双侧直径5 mm的标准骨缺损(n=18),随机分为3组:实验组植入HIF-1α-BMSCs/PLGA复合体(n=6),对照组植入BMSCs/PLGA复合体(n=6),材料组仅植入PLGA支架材料(n=6)。术后8周处死大鼠取材,分别行大体观察、X线片检查和HE染色观察缺损区骨形成情况。结果大体观察、X线片检查和HE染色均显示实验组缺损区新骨形成量明显大于对照组及材料组,差异有统计学意义(P<0.05)。结论体内研究表明HIF-1α能够介导BMSCs促进骨组织形成,PLGA是理想的支架材料,用其构建的血管化工程骨能够有效修复骨缺损。Objective To construct the vascular tissue engineering bone of hypoxia-inducible factor-1α （HIF-1α） mediated bone mesenchymal stem cells （BMSCs） combined with polylactie-co-glycolicacid（PLGA） scaffold, and to observe the effect on repairing the critical-sizedcalvarial bone defect in rat. Methods The target gene HIF-1α was transfected into BMSCs, and combined with PLGA scaffold, then implanted to repair bilateralealvarial defect （Ф 5 mm） of SD rat （n = 18）. The rats were randomly divided into three groups： HIF-1α-BMSCs/PLGA group （n = 6）, BMSCs/PLGA group （n = 6 ） and only PLGA scaffold group （n = 6 ）. The new bone formation was ob- served by gross inspection, X-ray examination and HE staining respectively at 8 weeks after surgery. Results The gross inspection, X-ray examination and HE staining showed that the amount of new bone formation in the experi- mental group was significantly higher than that in the control group and the material group, and the difference was statistically significant （P 〈 0. 05 ）. Conclusion HIF- 1can mediate BMSCs to promote bone tissue formation. PL- GA is an ideal scaffold material and the bone defect can be repaired effectively by using this vascular tissue engi- neering bone.

关 键 词：低氧诱导因子-1Α 骨髓间充质干细胞 聚乳酸-聚乙醇酸共聚物 组织工程骨 标准骨缺损 

分 类 号：R318.08[医药卫生—生物医学工程]