西格列汀对高糖条件下施万细胞增殖及3-NT含量的影响  

Effects of sitagliptin on proliferation and contents of 3-NT in Schwann cells under high glucose

作　　者：徐丹丹[1] 任明山[1] 吴元波[1] 朱翔[2] 江艳 鲁智敏 

机构地区：[1]安徽医科大学附属省立医院神经内科,合肥230001 [2]安徽医科大学附属省立医院干部病房,合肥230001 [3]安徽医科大学附属省立医院脑研究所,合肥230001 [4]安徽医科大学,临床医学合肥230601

出　　处：《安徽医科大学学报》2017年第9期1298-1302,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1608085MH209)

摘　　要：目的观察二肽基肽酶-Ⅳ(DPP-Ⅳ)抑制剂西格列汀对高糖条件下大鼠施万细胞(RSC96)增殖、凋亡及3-硝基酪氨酸(3-NT)含量的影响。方法通过体外培养RSC96细胞,建立高糖条件下D-葡萄糖100 mmol/L RSC96细胞模型,予以过氧亚硝基阴离子(ONOO-)抑制剂尿酸1μmol/L及西格列汀1μmol/L刺激48 h后,应用CCK-8及流式细胞技术观察尿酸及西格列汀对高糖条件下RSC96细胞增殖及细胞凋亡的影响。运用ELISA法检测尿酸及西格列汀对RSC96细胞内3-NT蛋白表达的影响。结果与正常对照组比较,高糖组抑制细胞增殖,促进细胞凋亡及胞内3-NT蛋白表达(P<0.05);尿酸及西格列汀可促进高糖条件下RSC96细胞增殖,抑制细胞凋亡及胞内3-NT蛋白表达(P<0.05)。结论西格列汀可能通过抑制细胞亚硝基化应激,从而增加高糖条件下RSC96细胞的增殖活性,抑制细胞凋亡,减轻高浓度葡萄糖对RSC96细胞的损伤。Objective To proliferation, apoptosis an ods Sehwann cell model model were treated with D μmol/L） for 48 hours, cose were observed by CCK-8 and flow cytometry. The effects of UA and sitagliptin on the expression of 3-NT pro- tein in RSC96 were detected by ELISA. Results Compared with normal control group, the hyperglycemia group in- hibited cell proliferation, promoted cell apoptosis and expression of intracellular 3-NT protein （ P 〈 0. 05 ） ; UA and sitagliptin could promote the proliferation of RSC96 under high glucose,inhibited apoptosis and intracellular 3-NT protein expression （P 〈 0. 05 ）. Conclusion Sitagliptin can increase the proliferation activity of RSC96 under high glucose, inhibit apoptosis and reduce the damage of RSC96 cells induced by high concentration of glucose by inhib- iting the nitrosylation stress of cells.

关 键 词：施万细胞 糖尿病周围神经病变 西格列汀 3-硝基酪氨酸 亚硝基化应激 

分 类 号：R285[医药卫生—中药学]