动脉粥样硬化中Toll样受体3上调β型干扰素的表达  

Toll-like receptor 3 increase the expression of interferon-β in atherosclerosis

作　　者：万立松 胡何节[1] 方征东[1] 王晓天[1] 孙小杰[1] 葛新宝[1] 程灿[1] 

机构地区：[1]安徽医科大学附属省立医院普外科,合肥230001

出　　处：《安徽医科大学学报》2017年第9期1314-1317,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1408085MH177)

摘　　要：目的观察动脉粥样硬化病变中Toll样受体3(TLR3)与β型干扰素(IFN-β)表达的关系及影响。方法下肢动脉硬化性闭塞症(ASO)患者20例,健康对照组20例,通过流式细胞术检测外周血单核细胞TLR3及IFN-β的表达。高脂饮食喂养Apo E(-/-)小鼠,实验组使用聚肌胞苷酸Poly(I:C)腹腔注射,对照组注射等剂量生理盐水。两日注射一次,经10周后,生化分析仪检测血浆中低密度脂蛋白胆固醇、高密度脂蛋白胆固醇、三酰甘油、总胆固醇、葡萄糖(Glu)水平,ELISA法检测血浆及动脉中TLR3、干扰素调节因子3(IRF3)、IFN-β及白介素-1β(IL-1β)水平。结果下肢ASO患者外周血单核细胞TLR3及IFN-β在ASO组的表达阳性率均显著高于对照组;Apo E(-/-)小鼠中,实验组血浆中Glu水平较对照组显著降低;实验组血浆及动脉中TLR3、IRF3、IFN-β水平较对照组显著增加,IL-1β水平较对照组显著降低。结论动脉粥样硬化病变中TLR3表达的增加诱导IFN-β,并通过下调IL-1β水平达到抗动脉粥样硬化的作用。Objective To investigate the relationship and influence of Toll-like receptor 3 （TLR3） and interferon-β （IFN-β） expression in atherosclerotic lesions. Methods In human subject, 40 subjects were enrolled. All sub- jects were divided into two groups：patients with arteriosclerosis obliterans（ASO） （n = 20）, control group （n = 20）. The positive rates of TLR3 and IFN-β in human peripheral blood monocytes were measured by flow cytometry. ApoE（ -/-） mice were fed in high-fat diet. The mice in experimental group were injected intraperitoneal（ip） by Po- ly （I：C） , The mice in control group were injected ip by normal saline. Every dose was injected once every two days for 10 weeks. Levels of high-density lipoprotein-cholesterol （ HDL-C ）, low-density lipoprotein-cholesterol （ LDL- C ）, triglyceride （ TG）, total cholesterol （ TC ）, glucose （Glu） in plasma were tested by biochemical analyzer. Lev- els of TLR3 ,IRF3, IFN-β and interleukin-1β（ IL-1 β） in plasma and aorta were tested by ELISA method. Results The expression rates of TLR3 and IFN-βin ASO group were significantly higher than those in control group. In ApoE（ -/-） mice, level of Glu in plasma in experimental group was significantly lower than that in control group, level of TLR3, IRF3, IFN-β in plasma and aorta in experimental group were significantly higher than those in control group, level of IL-1β in plasma and aorta in experimental group was significantly lower than that in control group. Conclusion The increased expression of TLR3 in as induced expression of IFN-β, which achieved the role of anti- arteriosclerosis by down-regulating the level of IL-1β.

关 键 词：动脉粥样硬化 单核细胞 TOLL样受体3 β型干扰素 

分 类 号：R654.3[医药卫生—外科学]