miR-18a调节HTR-8细胞中雌激素受体α表达的研究  

Effect of PE Related miR-18a on Expression of Estrogen Receptor Alpha in HTR-8 Cells

作　　者：杨洋[1] 徐妍妍[1] 马媛[2] 

机构地区：[1]西安医学院第一附属医院,陕西西安710077 [2]第四军医大学唐都医院,陕西西安710038

出　　处：《实用妇产科杂志》2017年第8期599-602,共4页Journal of Practical Obstetrics and Gynecology

摘　　要：目的:研究微小RNA-18a(miR-18a)在人正常滋养细胞(HTR8)中对雌激素受体α(ERα)表达的调控作用。方法:miR-18a前体分子[miR-18a类似物(miR-18a mimics)、miR-18a空白载体、miR-18a抑制物(miR-18a inhibitor)]转染HTR-8细胞,分为实验组1、阴性对照组(NC组)、实验组2。RT-PCR检测转染后各组中miR-18a的表达情况;RT-PCR与Western-blot检测HTR-8细胞中ERα在mRNA和蛋白水平的表达情况。结果:实验组1细胞中miR-18a的表达与NC组(0.407±0.019)相比明显增高(0.880±0.060),实验组2细胞中miR-18a的表达显著降低(0.160±0.014),差异有统计学意义(P<0.05)。转染48小时后实验组1ERαmRNA的表达(0.249±0.003)与NC组(0.313±0.010)相比差异无统计学意义(P>0.05);实验组2 ERαmRNA的表达水平明显增高(0.823±0.023),差异有统计学意义(P<0.05)。实验组2 ERα蛋白表达水平(1.030±0.006)与NC组(0.960±0.008)相比有增高,实验组1 ERα蛋白表达水平明显降低(0.660±0.013),差异有统计学意义(P<0.05)。结论:在人正常滋养细胞中,miR-18a可能在转录和翻译水平均对ERα具有调控作用。Objective：To study the effect of miR-18 a on expression of estrogen receptor alpha in human normal trophoblast cells（HTR-8）.Methods：The synthesized miR-18a（miR-18 amimics,miR-18 a inhibitor and blank carrier） were transfected into HTR-8 cells.Experiment included experimental group 1,pre-miRNA un-transfection group（NC group） and experimental group 3.RT-PCR detected the expression of miR-18 a in the three groups.RT-PCR and Western-blot detected the expression of ERα mRNA and protein in the transfected HTR-8 cells.Results：According to the results of RT-PCR,the expressions of miR-18 a in experimental group 1（0.880 ± 0.060）was significantly higher than that of NC group（0.407±0.019）（P0.05）,and the expressions of miR-18 a in experimental group 3（0.160±0.014） was significantly lower than that of NC group（0.407±0.019）（P0.05）;After transfected for 48 hours,there was no significant difference between the expression of ERα mRNA between experimental group 1（0.249±0.003） and NC group（0.313 ± 0.010）（P 0.05）,the expression of ERα mRNA in transfected cells in experimental group 3（0.823 ± 0.023） was significantly higher than that of NC group（P 0.05）;Western-blot results showed that the expression of ERα protein of experimental group 3（1.030±0.006）was significantly higher than that of NC group（0.960±0.008）（P0.05）;the expression of ERα protein of experimental group 1（0.660±0.013） was significantly lower than that of NC group（0.960±0.008）（P0.05）.Conclusions：In human normal trophoblast cells,miR-18 a may play regulatory role in the expression of ERα both in mRNA and protein levels.

关 键 词：微小RNA 滋养细胞 雌激素受体 子痫前期 

分 类 号：R714[医药卫生—妇产科学]